Idioma:

Prolonged inflammation leads to ongoing damage after spinal cord injury

Kwiecien, Jacek M ; Dabrowski, Wojciech ; Dąbrowska-Bouta, Beata ; Sulkowski, Grzegorz ; Oakden, Wendy ; Kwiecien-Delaney, Christian J ; Yaron, Jordan R ; Zhang, Liqiang ; Schutz, Lauren ; Marzec-Kotarska, Barbara ; Stanisz, Greg J ; Karis, John P ; Struzynska, Lidia ; Lucas, Alexandra R Nógrádi, Antal

PloS one, 2020, Vol.15 (3), p.e0226584-e0226584 [Periódico revisado por pares]

United States: Public Library of Science

Texto completo disponível

Citações Citado por

Enviar para

Título:
Prolonged inflammation leads to ongoing damage after spinal cord injury
Autor: Kwiecien, Jacek M ; Dabrowski, Wojciech ; Dąbrowska-Bouta, Beata ; Sulkowski, Grzegorz ; Oakden, Wendy ; Kwiecien-Delaney, Christian J ; Yaron, Jordan R ; Zhang, Liqiang ; Schutz, Lauren ; Marzec-Kotarska, Barbara ; Stanisz, Greg J ; Karis, John P ; Struzynska, Lidia ; Lucas, Alexandra R
Nógrádi, Antal
Assuntos: Animals ; Anti-Inflammatory Agents ; Arachnoiditis ; Arachnoiditis - diagnosis ; Arachnoiditis - immunology ; Arachnoiditis - pathology ; Astrocytes ; Astrocytes - immunology ; Astrocytes - metabolism ; Biology and Life Sciences ; CD163 antigen ; Chemokines ; Cytokines ; Cytokines - immunology ; Cytokines - metabolism ; Damage ; Disease Models, Animal ; Edema ; Glial cells ; Gliosis ; Gliosis - diagnosis ; Gliosis - immunology ; Gliosis - pathology ; Histopathology ; Humans ; IL-1β ; Immunotherapy ; Inflammation ; Injury prevention ; Interleukin 1 ; Laboratories ; Macrophages ; Macrophages - immunology ; Macrophages - metabolism ; Magnetic Resonance Imaging ; Male ; Medical research ; Medicine and Health Sciences ; Myelin ; Myelin Sheath - immunology ; Myelin Sheath - pathology ; Neuroprotection ; Pathogenesis ; Phagocytosis ; Physical sciences ; Proteomics ; Rats ; Research and Analysis Methods ; Severity of Illness Index ; Spinal Cord - cytology ; Spinal Cord - diagnostic imaging ; Spinal Cord - immunology ; Spinal Cord - pathology ; Spinal cord injuries ; Spinal Cord Injuries - complications ; Spinal Cord Injuries - diagnostic imaging ; Spinal Cord Injuries - immunology ; Spinal Cord Injuries - pathology ; Studies ; Time Factors ; Trauma ; Tumor necrosis factor ; Tumor necrosis factor-TNF ; Vaccines ; γ-Interferon
É parte de: PloS one, 2020, Vol.15 (3), p.e0226584-e0226584
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Descrição: The pathogenesis of spinal cord injury (SCI) remains poorly understood and treatment remains limited. Emerging evidence indicates that post-SCI inflammation is severe but the role of reactive astrogliosis not well understood given its implication in ongoing inflammation as damaging or neuroprotective. We have completed an extensive systematic study with MRI, histopathology, proteomics and ELISA analyses designed to further define the severe protracted and damaging inflammation after SCI in a rat model. We have identified 3 distinct phases of SCI: acute (first 2 days), inflammatory (starting day 3) and resolution (>3 months) in 16 weeks follow up. Actively phagocytizing, CD68+/CD163- macrophages infiltrate myelin-rich necrotic areas converting them into cavities of injury (COI) when deep in the spinal cord. Alternatively, superficial SCI areas are infiltrated by granulomatous tissue, or arachnoiditis where glial cells are obliterated. In the COI, CD68+/CD163- macrophage numbers reach a maximum in the first 4 weeks and then decline. Myelin phagocytosis is present at 16 weeks indicating ongoing inflammatory damage. The COI and arachnoiditis are defined by a wall of progressively hypertrophied astrocytes. MR imaging indicates persistent spinal cord edema that is linked to the severity of inflammation. Microhemorrhages in the spinal cord around the lesion are eliminated, presumably by reactive astrocytes within the first week post-injury. Acutely increased levels of TNF-alpha, IL-1beta, IFN-gamma and other pro-inflammatory cytokines, chemokines and proteases decrease and anti-inflammatory cytokines increase in later phases. In this study we elucidated a number of fundamental mechanisms in pathogenesis of SCI and have demonstrated a close association between progressive astrogliosis and reduction in the severity of inflammation.
Editor: United States: Public Library of Science
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Prolonged inflammation leads to ongoing damage after spinal cord injury

United States: Public Library of Science

Buscando em bases de dados remotas. Favor aguardar.