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Pericentromeric heterochromatin is hierarchically organized and spatially contacts H3K9me2 islands in euchromatin

Lee, Yuh Chwen G ; Ogiyama, Yuki ; Martins, Nuno M C ; Beliveau, Brian J ; Acevedo, David ; Wu, C-Ting ; Cavalli, Giacomo ; Karpen, Gary H Bosco, Giovanni

PLoS genetics, 2020-03, Vol.16 (3), p.e1008673-e1008673 [Periódico revisado por pares]

United States: Public Library of Science

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  • Título:
    Pericentromeric heterochromatin is hierarchically organized and spatially contacts H3K9me2 islands in euchromatin
  • Autor: Lee, Yuh Chwen G ; Ogiyama, Yuki ; Martins, Nuno M C ; Beliveau, Brian J ; Acevedo, David ; Wu, C-Ting ; Cavalli, Giacomo ; Karpen, Gary H
  • Bosco, Giovanni
  • Assuntos: Analysis ; Biology ; Biology and Life Sciences ; Chromosomes ; Cytogenetics ; Deoxyribonucleic acid ; DNA ; DNA methylation ; Drosophila ; Earth Sciences ; Euchromatin ; Funding ; Genes ; Genetics & Heredity ; Genomes ; Genomics ; Heterochromatin ; Laboratories ; Life Sciences ; Novels ; Proteins ; Research and Analysis Methods
  • É parte de: PLoS genetics, 2020-03, Vol.16 (3), p.e1008673-e1008673
  • Notas: new_version
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    PMCID: PMC7147806
    Japan Society for the Promotion of Science (JSPS)
    USDOE Office of Science (SC)
    European Union’s Horizon 2020 Research and Innovation Program
    Centre national de la recherche scientifique (CNRS)
    Damon Runyon Cancer Research Fellowship
    AC02-05CH11231; K99 GM121868; DP1GM106412; RO1HD091797; RO1GM123289; ERC-2008-AdG no. 232947; 676556; ANR-15-CE12-006-01; R01 GM117420
    National Institutes of Health (NIH)
    Agence Nationale de ala Recherche
    European Research Council (ERC)
    Current address: Department of Ecology and Evolutionary Biology, UC Irvine, Irvine, California, United States of America
    Harvard University has filed patent applications on behalf of C-tW pertaining to Oligopaints and related oligo-based methods for genome imaging.
  • Descrição: Membraneless pericentromeric heterochromatin (PCH) domains play vital roles in chromosome dynamics and genome stability. However, our current understanding of 3D genome organization does not include PCH domains because of technical challenges associated with repetitive sequences enriched in PCH genomic regions. We investigated the 3D architecture of Drosophila melanogaster PCH domains and their spatial associations with the euchromatic genome by developing a novel analysis method that incorporates genome-wide Hi-C reads originating from PCH DNA. Combined with cytogenetic analysis, we reveal a hierarchical organization of the PCH domains into distinct "territories." Strikingly, H3K9me2-enriched regions embedded in the euchromatic genome show prevalent 3D interactions with the PCH domain. These spatial contacts require H3K9me2 enrichment, are likely mediated by liquid-liquid phase separation, and may influence organismal fitness. Our findings have important implications for how PCH architecture influences the function and evolution of both repetitive heterochromatin and the gene-rich euchromatin.
  • Editor: United States: Public Library of Science
  • Idioma: Inglês
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