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Retinopathy with central oedema in an INS ^sup C94Y^ transgenic pig model of long-term diabetes

Kleinwort, Kristina J; H ; Amann, Barbara ; Hauck, Stefanie M ; Hirmer, Sieglinde ; Blutke, Andreas ; Renner, Simone ; Uhl, Patrizia B ; Lutterberg, Karina ; Sekundo, Walter ; Wolf, Eckhard ; Deeg, Cornelia A

Diabetologia, 2017-08, Vol.60 (8), p.1541 [Periódico revisado por pares]

Heidelberg: Springer Nature B.V

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  • Título:
    Retinopathy with central oedema in an INS ^sup C94Y^ transgenic pig model of long-term diabetes
  • Autor: Kleinwort, Kristina J; H ; Amann, Barbara ; Hauck, Stefanie M ; Hirmer, Sieglinde ; Blutke, Andreas ; Renner, Simone ; Uhl, Patrizia B ; Lutterberg, Karina ; Sekundo, Walter ; Wolf, Eckhard ; Deeg, Cornelia A
  • Assuntos: Animal models ; Antibodies ; Blindness ; Cataracts ; Diabetes ; Diabetes mellitus ; Diabetic retinopathy ; Edema ; Immunohistochemistry ; Microvasculature ; Retina ; Retinopathy ; Therapeutic applications
  • É parte de: Diabetologia, 2017-08, Vol.60 (8), p.1541
  • Descrição: Aims/hypothesis Diabetic retinopathy is a severe complication of diabetes mellitus that often leads to blindness. Because the pathophysiology of diabetic retinopathy is not fully understood and novel therapeutic interventions require testing, there is a need for reliable animal models that mimic all the complications of diabetic retinopathy. Pig eyes share important anatomical and physiological similarities with human eyes. Previous studies have demonstrated that INS C94Y transgenic pigs develop a stable diabetic phenotype and ocular alterations such as cataracts. The aim of this study was to conduct an in-depth analysis of pathological changes in retinas from INS C94Y pigs exposed to hyperglycaemia for more than 2 years, representing a chronic diabetic condition. Methods Eyes from six INS C94Ypigs and six age-matched control littermates were analysed via histology and immunohistochemistry. For histological analyses of retinal (layer) thickness, sections were stained with H&E or Mallory's trichrome. For comparison of protein expression patterns and vessel courses, sections were stained with different antibodies in immunohistochemistry. Observed lesions were compared with reported pathologies in human diabetic retinopathy. Results INS C94Ypigs developed several signs of diabetic retinopathy similar to those seen in humans, such as intraretinal microvascular abnormalities, symptoms of proliferative diabetic retinopathy and central retinal oedema in a region that is cone rich, like the human macula. Conclusions/interpretation The INS C94Ypig is an interesting model for studying the pathophysiology of diabetic retinopathy and for testing novel therapeutic strategies.
  • Editor: Heidelberg: Springer Nature B.V
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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