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On the Stability of DNA Origami Nanostructures in Low‐Magnesium Buffers

Kielar, Charlotte ; Xin, Yang ; Shen, Boxuan ; Kostiainen, Mauri A. ; Grundmeier, Guido ; Linko, Veikko ; Keller, Adrian

Angewandte Chemie, 2018-07, Vol.130 (30), p.9614-9618 [Peer Reviewed Journal]

Weinheim: Wiley Subscription Services, Inc

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  • Title:
    On the Stability of DNA Origami Nanostructures in Low‐Magnesium Buffers
  • Author: Kielar, Charlotte ; Xin, Yang ; Shen, Boxuan ; Kostiainen, Mauri A. ; Grundmeier, Guido ; Linko, Veikko ; Keller, Adrian
  • Subjects: Biomedical materials ; Buffers ; Chemistry ; Denaturation ; Deoxyribonucleic acid ; DNA ; DNA-Nanotechnologie ; DNA-Origami ; Ethylenediaminetetraacetic acids ; Ions ; Magnesium ; Nanostructure ; Rasterkraftmikroskopie ; Stabilität ; Structural integrity
  • Is Part Of: Angewandte Chemie, 2018-07, Vol.130 (30), p.9614-9618
  • Notes: These authors contributed equally to this work.
  • Description: DNA origami structures have great potential as functional platforms in various biomedical applications. Many applications, however, are incompatible with the high Mg2+ concentrations commonly believed to be a prerequisite for maintaining DNA origami integrity. Herein, we investigate DNA origami stability in low‐Mg2+ buffers. DNA origami stability is found to crucially depend on the availability of residual Mg2+ ions for screening electrostatic repulsion. The presence of EDTA and phosphate ions may thus facilitate DNA origami denaturation by displacing Mg2+ ions from the DNA backbone and reducing the strength of the Mg2+–DNA interaction, respectively. Most remarkably, these buffer dependencies are affected by DNA origami superstructure. However, by rationally selecting buffer components and considering superstructure‐dependent effects, the structural integrity of a given DNA origami nanostructure can be maintained in conventional buffers even at Mg2+ concentrations in the low‐micromolar range. DNA‐Origami‐Nanostrukturen wurden hinsichtlich ihrer Stabilität in verschiedenen Tris‐ und Phosphatpuffern bei Mg2+‐Konzentrationen im niedrigen mikromolaren Bereich untersucht. Ihre Stabilität wurde durch EDTA‐ und Phosphat‐Ionen gesenkt, die Mg2+‐Ionen vom DNA‐Rückgrat abziehen bzw. die Mg2+‐DNA‐Wechselwirkung schwächen. Diese Effekte waren für verschiedene DNA‐Origami‐Überstrukturen unterschiedlich ausgeprägt.
  • Publisher: Weinheim: Wiley Subscription Services, Inc
  • Language: English
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