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Wistar rat dermis recellularization

Martins, A.R. ; Matias, G.S.S. ; Batista, V.F. ; Miglino, M.A. ; Fratini, P.

Research in veterinary science, 2020-08, Vol.131, p.222-231 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
Wistar rat dermis recellularization
Autor: Martins, A.R. ; Matias, G.S.S. ; Batista, V.F. ; Miglino, M.A. ; Fratini, P.
Assuntos: Adipose tissue ; Alcohol ; Animal tissues ; Animals ; Bioengineering ; Biomedical materials ; Carcinoma ; Connective tissue ; Decellularization ; Deoxyribonucleic acid ; Dermis ; DNA ; Extracellular matrix ; Fibroblasts ; Genomics ; Graft rejection ; Immunofluorescence ; Lesions ; Medicine ; Mesenchyme ; Recellularization ; Scaffold ; Scaffolds ; Scanning electron microscopy ; Skin ; Skin diseases ; Skin lesions ; Stem cell transplantation ; Stem cells ; Sterilization ; Tissue engineering ; Transplantation ; Veterinary medicine
É parte de: Research in veterinary science, 2020-08, Vol.131, p.222-231
Descrição: Skin lesions are normal to all species, regardless of gender or age. The skin, the largest organ of the body, has function as a primary barrier to the chemical, physical and biological aggressions of the environment. In animals, these lesions may be due to fights and/or predations, also as in humans, there is a very common cause of dermal lesions that are caused by burns and carcinomas. Looking for new techniques of tissue bioengineering, studies have been shown promising results for formulations of acellular biological scaffolds from tissue decellularization for the reconstitution of these lesions. The decellularization has its proof by a varied range of tests such as scanning electron microscopy and residual genomic DNA tests. Subsequently the tissue can go through the process of recellularization using cells of interest, even the animal that will receive this tissue, reducing the risks of rejection and improving the response to tissue transplantation. Thus, this manuscript aimed at the decellularization of the tissue with the use of chemical and physical means followed by sterilization and the establishment of a protocol for the recellularization of a decellularized scaffold from the Wistar rat dermis using murine fibroblasts and mesenchymal stem cells from canine adipose tissue for 7 days. After efficacy tests, the tissue recellularization were confirmed by immunofluorescence assays and scanning electron microscopy where the adherence of the cells in the biological scaffold was observed. •Architecture and proteins of ECM was preserved in decellularized dermis to recellularization process;•A viable biological scaffold in the use for grafting in regenerative medicine;•A cell-matrix integration capability with stem cells making it a new tool available for reconstructive surgeries.
Editor: England: Elsevier Ltd
Idioma: Inglês

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Wistar rat dermis recellularization

Martins, A.R. ; Matias, G.S.S. ; Batista, V.F. ; Miglino, M.A. ; Fratini, P.

England: Elsevier Ltd

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