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Creation and identification of peanut germplasm tolerant to triazolopyrimidine herbicides

LIU, Jia-Xin ; LAN, Yu ; XU, Qian-Yu ; LI, Hong-Ye ; ZHOU, Xin-Yu ; ZHAO, Xuan ; GAN, Yi ; LIU, Hong-Bo ; ZHENG, Yue-Ping ; ZHAN, Yi-Hua ; ZHANG, Gang ; ZHENG, Zhi-Fu

Zuo wu xue bao, 2022-04, Vol.48 (4), p.1027-1034 [Periódico revisado por pares]

Beijing: Chinese Academy of Agricultural Sciences (CAAS)

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  • Título:
    Creation and identification of peanut germplasm tolerant to triazolopyrimidine herbicides
  • Autor: LIU, Jia-Xin ; LAN, Yu ; XU, Qian-Yu ; LI, Hong-Ye ; ZHOU, Xin-Yu ; ZHAO, Xuan ; GAN, Yi ; LIU, Hong-Bo ; ZHENG, Yue-Ping ; ZHAN, Yi-Hua ; ZHANG, Gang ; ZHENG, Zhi-Fu
  • Assuntos: AHAS gene ; Amino acid sequence ; Amino acids ; Chromosomes ; Cloning ; Ethyl methanesulfonate ; Gene expression ; Gene sequencing ; Germplasm ; Herbicide resistance ; Herbicides ; Mutagenesis ; Mutants ; Nucleotides ; Peanuts ; Sequence analysis
  • É parte de: Zuo wu xue bao, 2022-04, Vol.48 (4), p.1027-1034
  • Descrição: The current shortage of herbicide-tolerant peanut germplasm resources restricts the diversified development of peanut cultivation models. In order to create peanut germplasm resources resistant to different herbicides, we used ethyl methanesulfonate mutagenesis technology to create a A mutagenized population consisting of more than 55,000 peanut lines was then screened with a variety of different herbicides to obtain multiple peanut mutants that were tolerant to different herbicides. One of the mutants was foliar sprayed in the field Strong tolerance to fenoxsulam and xoxsulam was shown in herbicide tolerance evaluation tests under various laboratory conditions, but this tolerance trait did not affect peanut yield and peanut yield. To determine whether this trait is associated with herbicide target resistance, we compared and analyzed the gene sequences of the two herbicide target enzymes (acetohydroxy acid synthase, AHAS) in the mutant and the wild type. Molecular cloning and sequence analysis showed that each of peanut A10 and B10 chromosomes contained a gene highly similar to Arabidopsis AtAHAS sequence, named AhAHAS1a and AhAHAS1b respectively. Peanut A08 and B08 chromosomes also contained An AHAS gene, named AhAHAS2a and AhAHAS2b, respectively. However, compared with the wild type, these genes in the mutant did not have nucleotide substitutions that could change the amino acid sequence. Furthermore, it was found that the expression of the AhAHAS gene was significantly increased in the mutant. There were no significant differences between the peanut mutant and the wild type. These results suggest that the herbicide tolerance of this peanut mutant may be caused by a non-target resistance mechanism.
  • Editor: Beijing: Chinese Academy of Agricultural Sciences (CAAS)
  • Idioma: Inglês;Chinês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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