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Canine Fibroblasts Expressing Human Transcription Factors: What is in the Route for the Production of Canine Induced Pluripotent Stem Cells

Gonçalves, NJN ; Bressan, FF ; Souza, A ; Martins, DS ; Miglino, MA ; Meirelles, FV ; Perecin, F ; Ambrósio, CE

Reproduction in domestic animals, 2012-12, Vol.47 (s6), p.84-87 [Peer Reviewed Journal]

Germany: Blackwell Publishing Ltd

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Title:
Canine Fibroblasts Expressing Human Transcription Factors: What is in the Route for the Production of Canine Induced Pluripotent Stem Cells
Author: Gonçalves, NJN ; Bressan, FF ; Souza, A ; Martins, DS ; Miglino, MA ; Meirelles, FV ; Perecin, F ; Ambrósio, CE
Subjects: Animals ; Cell Culture Techniques ; Dogs ; Dogs - physiology ; Fibroblasts - metabolism ; Flow Cytometry ; Gene expression ; Gene Expression Regulation - physiology ; Humans ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - physiology ; Microscopy, Confocal ; Octamer Transcription Factor-3 - genetics ; Octamer Transcription Factor-3 - metabolism ; SOXB1 Transcription Factors - genetics ; SOXB1 Transcription Factors - metabolism ; Species Specificity ; Stem cells
Is Part Of: Reproduction in domestic animals, 2012-12, Vol.47 (s6), p.84-87
Notes: State of São Paulo Research Foundation - No. 2012/01060-4
ark:/67375/WNG-M4BKRRTB-K
FAPESP graduate fellowships - No. 2011/22915-5; No. 2009/11631-6
ArticleID:RDA12002
istex:B4DEB0E57BE21F3B380967B9AF38C6BD2CF1D8DA
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: Contents The aim of this study was to further clarify the mechanisms involved in inducing pluripotency using canine foetal fibroblast cells. The two pluripotency‐related transcription factors, OCT4 and SOX2, coupled to a fluorescent reporter gene were transduced, individually or in combination, using a lentiviral system. Stable transgenic cell lineages were obtained and canine cells showed to be highly responsive to the integration and expression of human SOX2 and OCT4, also depending on the amount of virus used for incubation. Such positive results are essential for the establishment of pluripotency induction through the incorporation of known transcription factors into the genome of somatic cells.
Publisher: Germany: Blackwell Publishing Ltd
Language: English

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Canine Fibroblasts Expressing Human Transcription Factors: What is in the Route for the Production of Canine Induced Pluripotent Stem Cells

Gonçalves, NJN ; Bressan, FF ; Souza, A ; Martins, DS ; Miglino, MA ; Meirelles, FV ; Perecin, F ; Ambrósio, CE

Germany: Blackwell Publishing Ltd

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