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Analysis of the molecular characteristics and cloning of full-length coding sequence of interleukin-2 in tree shrews

Huang, Xiao-Yan ; Li, Ming-Li ; Xu, Juan ; Gao, Yue-Dong ; Wang, Wen-Guang ; Yin, An-Guo ; Li, Xiao-Fei ; Sun, Xiao-Mei ; Xia, Xue-Shan ; Dai, Jie-Jie

Dōngwùxué yánjiū, 2013-04, Vol.34 (2), p.121-126 [Periódico revisado por pares]

China: Kunming Institute of Zoology, The Chinese Academy of Sciences

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Título:
Analysis of the molecular characteristics and cloning of full-length coding sequence of interleukin-2 in tree shrews
Autor: Huang, Xiao-Yan ; Li, Ming-Li ; Xu, Juan ; Gao, Yue-Dong ; Wang, Wen-Guang ; Yin, An-Guo ; Li, Xiao-Fei ; Sun, Xiao-Mei ; Xia, Xue-Shan ; Dai, Jie-Jie
Assuntos: Amino Acid Sequence ; Animals ; Base Sequence ; Cattle ; Cloning, Molecular ; Cytokines ; Humans ; Immune system ; Interleukin-2 - chemistry ; Interleukin-2 - genetics ; Macaca mulatta ; Mammals - classification ; Mammals - genetics ; Mice ; Models, Molecular ; Molecular Sequence Data ; Monoclonal antibodies ; Open Reading Frames ; Phylogenetics ; Phylogeny ; Rats ; Sequence Alignment ; Small mammals ; Tupaia ; Tupaia belangeri ; Tupaiidae - classification ; Tupaiidae - genetics
É parte de: Dōngwùxué yánjiū, 2013-04, Vol.34 (2), p.121-126
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Descrição: While the tree shrew (Tupaia belangeri chinensis) is an excellent animal model for studying the mechanisms of human diseases, but few studies examine interleukin-2 (IL-2), an important immune factor in disease model evaluation. In this study, a 465 bp of the full-length IL-2 cDNA encoding sequence was cloned from the RNA of tree shrew spleen lymphocytes, which were then cultivated and stimulated with ConA (concanavalin). Clustal W 2.0 was used to compare and analyze the sequence and molecular characteristics, and establish the similarity of the overall structure of IL-2 between tree shrews and other mammals. The homology of the IL-2 nucleotide sequence between tree shrews and humans was 93%, and the amino acid homology was 80%. The phylogenetic tree results, derived through the Neighbour-Joining method using MEGA5.0, indicated a close genetic relationship between tree shrews, Homo sapiens, and Macaca mulatta. The three-dimensional structure analysis showed that the surface charges in most regions of tree shrew IL-2 were similar to between tree shrews and humans; however, the N-glycosylation sites and local structures were different, which may affect antibody binding. These results provide a fundamental basis for the future study of IL-2 monoclonal antibody in tree shrews, thereby improving their utility as a model.
Editor: China: Kunming Institute of Zoology, The Chinese Academy of Sciences
Idioma: Chinês;Inglês

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Analysis of the molecular characteristics and cloning of full-length coding sequence of interleukin-2 in tree shrews

Huang, Xiao-Yan ; Li, Ming-Li ; Xu, Juan ; Gao, Yue-Dong ; Wang, Wen-Guang ; Yin, An-Guo ; Li, Xiao-Fei ; Sun, Xiao-Mei ; Xia, Xue-Shan ; Dai, Jie-Jie

China: Kunming Institute of Zoology, The Chinese Academy of Sciences

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