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Photosynthesis in enzymatically isolated leaf cells from the CAM plant Sedum telephium L

Spalding, M.H ; Edwards, G.E

Planta, 1978, Vol.141 (1), p.59-63 [Periódico revisado por pares]

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Título:
Photosynthesis in enzymatically isolated leaf cells from the CAM plant Sedum telephium L
Autor: Spalding, M.H ; Edwards, G.E
Assuntos: Acidity ; Cell separation ; Crassulacean acid metabolism ; Enzymes ; Leaves ; Mesophyll cells ; Photosynthesis ; Plant cells ; Plants ; Spinach
É parte de: Planta, 1978, Vol.141 (1), p.59-63
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: A technique has been developed for the enzymatic isolation of leaf cells from the Crassulacean acid-metabolism plant Sedum telephium. The cells exhibited high activity in both 14CO2 incorporation (30—70 μmol CO2 mg-1 chlorophyll h-1) and O2 evolution in the presence of bicarbonate (60—110 μmol O2 mg-1 chlorophyll h-1). Half-maximum saturation of 14CO2 incorporation occurred at a bicarbonate concentration of ca. 2 mM (20 μM CO2) at pH 8.4 and 30° C. Two types of light-dependent O2 evolution are reported: O2 evolution in the absence of exogenously supplied bicarbonate (endogenous O2 evolution), and bicarbonate-stimulated O2 evolution. Oxygen evolution in the presence of approximately ambient concentrations of CO2 appeared to be a combination of the endogenous O2 evolution and O2 evolution from fixation of the exogenously supplied CO2.
Editor: Germany: Springer-Verlag
Idioma: Inglês

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