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Production of alkaline protease with Bacillus licheniformis in a controlled fed-batch process

EBERHARD GIESECKE, U ; BIERBAUM, G ; RUDDE, H ; SPOHN, U ; WANDREY, C

Applied microbiology and biotechnology, 1991-09, Vol.35 (6), p.720-724 [Periódico revisado por pares]

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Título:
Production of alkaline protease with Bacillus licheniformis in a controlled fed-batch process
Autor: EBERHARD GIESECKE, U ; BIERBAUM, G ; RUDDE, H ; SPOHN, U ; WANDREY, C
Assuntos: Biological and medical sciences ; Biotechnology ; Enzyme engineering ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; Production of selected enzymes
É parte de: Applied microbiology and biotechnology, 1991-09, Vol.35 (6), p.720-724
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Descrição: The alkaline serine protease subtilisin Carlsberg is excreted into the medium by strains of Bacillus licheniformis or B. pumilus in the early stationary phase. A production method for alkaline serine protease with Bacillus licheniformis in a synthetic medium was developed. Employing closed-loop control of oxygen, nitrogen and carbon source the pO sub(2) was held at 5%, the ammonium concentration kept below 1 mM and the glycerol concentration was maintained between 20 and 100 mM. Protease production was monitored by flow injection analysis. Thus, in a fed-batch procedure production could be increased 4.6-fold in comparison to an uncontrolled batch process.
Editor: Berlin: Springer
Idioma: Inglês

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