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Impact of microRNA-134 on neural cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by targeting HSPA12B

Chi, Wenying ; Meng, Fanjun ; Li, Yan ; Li, Peilong ; Wang, Guizhi ; Cheng, Hong ; Han, Song ; Li, Junfa

Brain research, 2014-12, Vol.1592, p.22-33 [Peer Reviewed Journal]

Amsterdam: Elsevier B.V

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Title:
Impact of microRNA-134 on neural cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by targeting HSPA12B
Author: Chi, Wenying ; Meng, Fanjun ; Li, Yan ; Li, Peilong ; Wang, Guizhi ; Cheng, Hong ; Han, Song ; Li, Junfa
Subjects: Animals ; Biological and medical sciences ; Brain - pathology ; Brain - physiopathology ; Brain Ischemia - pathology ; Brain Ischemia - physiopathology ; Cell Death ; Cell Hypoxia - physiology ; Cell Survival - physiology ; Cells, Cultured ; Disease Models, Animal ; Fundamental and applied biological sciences. Psychology ; Glucose - deficiency ; Heat shock protein A12B (HSPA12B) ; HSP70 Heat-Shock Proteins - genetics ; HSP70 Heat-Shock Proteins - metabolism ; Infarction, Middle Cerebral Artery ; Ischemic stroke ; Male ; Medical sciences ; Mice, Inbred C57BL ; MicroRNA-134 (miR-134) ; MicroRNAs - antagonists & inhibitors ; MicroRNAs - metabolism ; Middle cerebral artery occlusion (MCAO) ; Neurology ; Neurons - pathology ; Neurons - physiology ; Oxygen-glucose deprivation (OGD) ; RNA, Small Interfering ; Stroke - pathology ; Stroke - physiopathology ; Vascular diseases and vascular malformations of the nervous system ; Vertebrates: nervous system and sense organs
Is Part Of: Brain research, 2014-12, Vol.1592, p.22-33
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: Abstract As a newly discovered member of the HSP70 family, heat shock protein A12B (HSPA12B) is involved in brain ischemic injury. According to our previous study, microRNA-134 (miR-134) could target HSPA12B by binding to its 3′-untranslated region (UTR). However, the regulation of miR-134 on HSPA12B and their role in protecting neuronal cells from ischemic injury are unclear. In this study, the miR-134 expression level was manipulated, and the HSPA12B protein levels were also determined in oxygen-glucose deprivation (OGD)-treated primary cultured neuronal cells in vitro and mouse brain after middle cerebral artery occlusion (MCAO)-induced ischemic stroke in vivo . The results showed that miR-134 expression levels increased in primary cultured neuronal cells and mouse brain from 12 h to 7 day reoxygenation/reperfusion after 1 h OGD or 1 h MCAO treatment. miR-134 overexpression promoted neuronal cell death and apoptosis by decreasing HSPA12B protein levels. Conversely, downregulating miR-134 reduced neuronal cell death and apoptosis by enhancing HSPA12B protein levels. Also, HSPA12B siRNA could block miR-134 inhibitor-mediated neuroprotection against OGD-induced neuronal cell injury in vitro . Taken together, miR-134 might influence neuronal cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by negatively modulating HSPA12B protein expression in a posttranscriptional manner.
Publisher: Amsterdam: Elsevier B.V
Language: English

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Impact of microRNA-134 on neural cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by targeting HSPA12B

Chi, Wenying ; Meng, Fanjun ; Li, Yan ; Li, Peilong ; Wang, Guizhi ; Cheng, Hong ; Han, Song ; Li, Junfa

Amsterdam: Elsevier B.V

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