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Growth on poly(l-lactic acid) porous scaffold preserves CD73 and CD90 immunophenotype markers of rat bone marrow mesenchymal stromal cells

Zamparelli, Alessandra ; Zini, Nicoletta ; Cattini, Luca ; Spaletta, Giulia ; Dallatana, Davide ; Bassi, Elena ; Barbaro, Fulvio ; Iafisco, Michele ; Mosca, Salvatore ; Parrilli, Annapaola ; Fini, Milena ; Giardino, Roberto ; Sandri, Monica ; Sprio, Simone ; Tampieri, Anna ; Maraldi, Nadir M. ; Toni, Roberto

Journal of materials science. Materials in medicine, 2014-10, Vol.25 (10), p.2421-2436 [Revista revisada por pares]

Boston: Springer US

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Growth on poly(l-lactic acid) porous scaffold preserves CD73 and CD90 immunophenotype markers of rat bone marrow mesenchymal stromal cells
Autor: Zamparelli, Alessandra ; Zini, Nicoletta ; Cattini, Luca ; Spaletta, Giulia ; Dallatana, Davide ; Bassi, Elena ; Barbaro, Fulvio ; Iafisco, Michele ; Mosca, Salvatore ; Parrilli, Annapaola ; Fini, Milena ; Giardino, Roberto ; Sandri, Monica ; Sprio, Simone ; Tampieri, Anna ; Maraldi, Nadir M. ; Toni, Roberto
Materias: 5'-Nucleotidase - metabolism ; Adhesion ; Animals ; Biological and medical sciences ; Biomarkers - metabolism ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biomedical materials ; Bone marrow ; Bone Marrow Cells - drug effects ; Bone Marrow Cells - metabolism ; Bone Marrow Cells - physiology ; Bones ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; Ceramics ; Chemistry and Materials Science ; Composites ; Genotype & phenotype ; Glass ; Human ; Immunophenotyping ; Lactic Acid - chemistry ; Lactic Acid - pharmacology ; Male ; Markers ; Materials Science ; Materials Testing ; Medical sciences ; Mesenchymal Stromal Cells - drug effects ; Mesenchymal Stromal Cells - metabolism ; Mesenchymal Stromal Cells - physiology ; Natural Materials ; Polyesters ; Polylactic acid ; Polymer Sciences ; Polymers - chemistry ; Polymers - pharmacology ; Porosity ; Rats ; Rats, Sprague-Dawley ; Regenerative Medicine/Tissue Engineering ; Scaffolds ; Surfaces and Interfaces ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgical implants ; Technology. Biomaterials. Equipments ; Thin Films ; Thy-1 Antigens - metabolism ; Tissue engineering ; Tissue Scaffolds - chemistry
Es parte de: Journal of materials science. Materials in medicine, 2014-10, Vol.25 (10), p.2421-2436
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descripción: Few data are available on the effect of biomaterials on surface antigens of mammalian bone marrow-derived, adult mesenchymal stromal cells (MSCs). Since poly( l -lactic acid) or PLLA is largely used in tissue engineering of human bones, and we are developing a reverse engineering program to prototype with biomaterials the vascular architecture of bones for their bioartificial reconstruction, both in humans and animal models, we have studied the effect of porous, flat and smooth PLLA scaffolds on the immunophenotype of in vitro grown, rat MSCs in the absence of any coating, co-polymeric enrichment, and differentiation stimuli. Similar to controls on plastic, we show that our PLLA scaffold does not modify the distribution of some surface markers in rat MSCs. In particular, the maintained expression of CD73 and CD90 on two different subpopulations (small and large cells) is consistent with their adhesion to the PLLA scaffold through specialized appendages, and to their prominent content in actin. In addition, our PLLA scaffold favours retention of the intermediate filament desmin, believed a putative marker of undifferentiated state. Finally, it preserves all rat MSCs morphotypes, and allows for their survival, adhesion to the substrate, and replication. Remarkably, a subpopulation of rat MSCs grown on our PLLA scaffold exhibited formation of membrane protrusions of uncertain significance, although in a size range and morphology compatible with either motility blebs or shedding vesicles. In summary, our PLLA scaffold has no detrimental effect on a number of features of rat MSCs, primarily the expression of CD73 and CD90.
Editor: Boston: Springer US
Idioma: Inglés

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Growth on poly(l-lactic acid) porous scaffold preserves CD73 and CD90 immunophenotype markers of rat bone marrow mesenchymal stromal cells

Boston: Springer US

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