Idioma:

Yeast-based self-organized hybrid bio-silica sol-gels for the design of biosensors

Ponamoreva, O N ; Kamanina, O A ; Alferov, V A ; Machulin, A V ; Rogova, T V ; Arlyapov, V A ; Alferov, S V ; Suzina, N E ; Ivanova, E P

Biosensors & bioelectronics, 2015-05, Vol.67, p.321-326 [Periódico revisado por pares]

England

Texto completo disponível

Citações Citado por

Enviar para

Título:
Yeast-based self-organized hybrid bio-silica sol-gels for the design of biosensors
Autor: Ponamoreva, O N ; Kamanina, O A ; Alferov, V A ; Machulin, A V ; Rogova, T V ; Arlyapov, V A ; Alferov, S V ; Suzina, N E ; Ivanova, E P
Assuntos: Biosensing Techniques ; Biosensors ; Catalysis ; Cryptococcus - chemistry ; Cryptococcus curvatus ; Encapsulation ; Methanol - isolation & purification ; Methyl alcohol ; Nucleation ; Pichia - chemistry ; Pichia angusta ; Polyethylene glycol ; Polyethylene Glycols - chemistry ; Polymethyl Methacrylate - chemistry ; Porosity ; Silanes - chemistry ; Silica Gel - chemistry ; Sol gel process ; Three dimensional ; Yeast
É parte de: Biosensors & bioelectronics, 2015-05, Vol.67, p.321-326
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The methylotrophic Pichia angusta VKM Y-2559 and the oleaginous Cryptococcus curvatus VKM Y-3288 yeast cells were immobilized in a bimodal silica-organic sol-gel matrix comprised of tetraethoxysilane (TEOS), the hydrophobic additive methyltriethoxysilane (MTES) and the porogen polyethylene glycol (PEG). Under carefully optimized experimental conditions, employing basic catalysts, yeast cells have become the nucleation centers for a silica-organic capsule assembled around the cells. The dynamic process involved in the formation of the sol-gel matrix has been investigated using optical and scanning electron microscopic techniques. The results demonstrated the influence of the MTES composition on the nature of the encapsulation of the yeast cells, together with the architecture of the three-dimensional (3D) sol-gel biomatrix that forms during the encapsulation process. A silica capsule was found to form around each yeast cell when using 85 vol% MTES. This capsule was found to protect the microorganisms from the harmful effects that result from exposure to heavy metal ions and UV radiation. The encapsulated P. angusta BKM Y-2559 cells were then employed as a biosensing element for the detection of methanol. The P. angusta-based biosensor is characterized by high reproducibility (Sr, 1%) and operational stability, where the biosensor remains viable for up to 28 days.
Editor: England
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 5 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Yeast-based self-organized hybrid bio-silica sol-gels for the design of biosensors

Ponamoreva, O N ; Kamanina, O A ; Alferov, V A ; Machulin, A V ; Rogova, T V ; Arlyapov, V A ; Alferov, S V ; Suzina, N E ; Ivanova, E P

England

Buscando em bases de dados remotas. Favor aguardar.