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Establishment and Cellular Characteristics of a Hepatocyte Cell Line (OUMS-31) Derived from an Acatalasemic Mouse

Asami Kondo ; Miyazaki, Masahiro ; Hong Pu ; Chong Gao ; Namba, Masayoshi

In vitro cellular & developmental biology. Animal, 1999-03, Vol.35 (3), p.155-158 [Periódico revisado por pares]

Germany: Society for In Vitro Biology

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Título:
Establishment and Cellular Characteristics of a Hepatocyte Cell Line (OUMS-31) Derived from an Acatalasemic Mouse
Autor: Asami Kondo ; Miyazaki, Masahiro ; Hong Pu ; Chong Gao ; Namba, Masayoshi
Assuntos: Acatalasia ; Albumins - genetics ; alpha-Fetoproteins - genetics ; Animals ; Catalase ; Catalase - genetics ; Catalase - metabolism ; Cattle ; Cell Line - enzymology ; Cell lines ; Cellular Models ; Collagen ; Collagenase ; Cultured cells ; Deoxyribonucleic acid ; DNA ; Epithelial cells ; Female ; Genetic mutation ; Glutathione ; Glutathione peroxidase ; Hep G2 cells ; Hepatocytes ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; Karyotyping ; Liver ; Liver - cytology ; Liver - enzymology ; Liver cells ; Mice ; Mice, Inbred C3H ; Mutation ; Nucleotide sequence ; Peroxidase ; Rodents ; Sequence analysis ; Tumor cell line ; Tumors
É parte de: In vitro cellular & developmental biology. Animal, 1999-03, Vol.35 (3), p.155-158
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Descrição: Liver cell lines with very low catalase activity were established from an acatalasemic mouse. Hepatocytes isolated by a collagenase-liver-perfusion technique were cultured in Williams' E medium supplemented with 10% fetal bovine serum. The acatalasemic liver cell line showed approximately 20% of the catalase activity of a normal mouse liver cell line, whereas its glutathione peroxidase activity was approximately equal to that of the normal liver cell line. DNA sequence analysis of this cell line showed the same mutation in the catalase gene as is seen in the acatalasemic mouse. Our observation of intracellular content of hydrogen peroxide (H2O 2) radical and increased susceptibility of the cells to H2O 2were compatible with the existence of low catalase activity in the acatalasemic mouse. This hepatocyte cell line should be useful for studying effects of oxidative radical stress at the cellular level.
Editor: Germany: Society for In Vitro Biology
Idioma: Inglês

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Establishment and Cellular Characteristics of a Hepatocyte Cell Line (OUMS-31) Derived from an Acatalasemic Mouse

Asami Kondo ; Miyazaki, Masahiro ; Hong Pu ; Chong Gao ; Namba, Masayoshi

Germany: Society for In Vitro Biology

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