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Sample injection and electrophoretic separation on a simple laminated paper based analytical device

Xu, Chunxiu ; Zhong, Minghua ; Cai, Longfei ; Zheng, Qingyu ; Zhang, Xiaojun

Electrophoresis, 2016-02, Vol.37 (3), p.476-481 [Periódico revisado por pares]

Germany: Verlag Chemie

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  • Título:
    Sample injection and electrophoretic separation on a simple laminated paper based analytical device
  • Autor: Xu, Chunxiu ; Zhong, Minghua ; Cai, Longfei ; Zheng, Qingyu ; Zhang, Xiaojun
  • Assuntos: Buffers ; Channels ; cutting ; Devices ; electrophoresis ; Electrophoresis - instrumentation ; Electrophoretic separation ; Equipment Design ; Hydrogen-Ion Concentration ; Laminated paper based analytical device ; Mathematical analysis ; Micro total analysis system ; Microfluidic Analytical Techniques - instrumentation ; Microfluidics ; mixing ; Sample injection ; Sampling ; Separation ; systems analysis ; wastes
  • É parte de: Electrophoresis, 2016-02, Vol.37 (3), p.476-481
  • Notas: http://dx.doi.org/10.1002/elps.201500321
    Guangdong Provincial Natural Science Foundation of China - No. S2013010012046
    Hanshan Normal University - No. QD20120521; No. QD20110616
    ark:/67375/WNG-Q06BJSKP-5
    ArticleID:ELPS5702
    istex:9A14C58A3CF1472B19337468F15B249486C82027
    See the article online to view Figs. 2 and 6 in colour.
    Colour Online
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: We described a strategy to perform multistep operations on a simple laminated paper‐based separation device by using electrokinetic flow to manipulate the fluids. A laminated crossed‐channel paper‐based separation device was fabricated by cutting a filter paper sheet followed by lamination. Multiple function units including sample loading, sample injection, and electrophoretic separation were integrated on a single paper based analytical device for the first time, by applying potential at different reservoirs for sample, sample waste, buffer, and buffer waste. As a proof‐of‐concept demonstration, mixed sample solution containing carmine and sunset yellow were loaded in the sampling channel, and then injected into separation channel followed by electrophoretic separation, by adjusting the potentials applied at the four terminals of sampling and separation channel. The effects of buffer pH, buffer concentration, channel width, and separation time on resolution of electrophoretic separation were studied. This strategy may be used to perform multistep operations such as reagent dilution, sample injection, mixing, reaction, and separation on a single microfluidic paper based analytical device, which is very attractive for building micro total analysis systems on microfluidic paper based analytical devices.
  • Editor: Germany: Verlag Chemie
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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