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Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator

Zhang, Xiaoyue ; Li, Yonghao ; Zhao, Xinqing ; Bai, Fengwu

Bioresource technology, 2017-01, Vol.223, p.317-322 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator
Autor: Zhang, Xiaoyue ; Li, Yonghao ; Zhao, Xinqing ; Bai, Fengwu
Assuntos: Artificial transcription factor ; Bioreactors - microbiology ; Carbon - metabolism ; Cellulase - genetics ; Cellulase - metabolism ; Cellulase production ; Fermentation - genetics ; Fungal Proteins - metabolism ; Glucose ; Glucose - metabolism ; Hydrolysis ; Organisms, Genetically Modified ; Transcription Factors - genetics ; Transcriptional Activation - genetics ; Transgenes ; Trichoderma - enzymology ; Trichoderma - genetics ; Trichoderma - metabolism ; Trichoderma reesei ; xyr1 and cre1 ; Zea mays - metabolism
É parte de: Bioresource technology, 2017-01, Vol.223, p.317-322
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: [Display omitted] •An artificial transcription activator xyr1-cre1b was expressed in T. reesei Rut C30.•The mutants displayed constitutive (hemi) cellulase production from glucose.•FPase of 2.63IU/mL was achieved within short time (48h) in a 7-L fermenter.•The crude enzyme is suitable for sugar release from alkali pretreated corn stover. The high cost of cellulase production presents biggest challenge in biomass deconstruction. Cellulase production by Trichoderma reesei using low cost carbon source is of great interest. In this study, an artificial transcription activator containing the Cre1 binding domain linked to the Xyr1 effector and binding domains was designed and constitutively overexpressed in T. reesei RUT C30. The recombinant strain T. reesei zxy-2 displayed constitutive cellulase production using glucose as a sole carbon source, and the production titer was 12.75-fold of that observed with T. reesei RUT C30 in shake flask culture. Moreover, FPase and xylanase titers of 2.63 and 108.72IU/mL, respectively, were achieved using glucose as sole carbon source within 48h in a 7-L fermenter by batch fermentation using T. reesei zxy-2. The crude enzyme obtained was used to hydrolyze alkali pretreated corn stover, and a high glucose yield of 99.18% was achieved.
Editor: England: Elsevier Ltd
Idioma: Inglês

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Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator

Zhang, Xiaoyue ; Li, Yonghao ; Zhao, Xinqing ; Bai, Fengwu

England: Elsevier Ltd

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