skip to main content
Idioma:
Tipo de recurso Mostra resultados com: Mostra resultados com: Índice

Vasopressin-dependent Inhibition of the C-type Natriuretic Peptide Receptor, NPR-B/GC-B, Requires Elevated Intracellular Calcium Concentrations

Abbey, Sarah E ; Potter, Lincoln R

The Journal of biological chemistry, 2002-11, Vol.277 (45), p.42423-42430 [Periódico revisado por pares]

United States: American Society for Biochemistry and Molecular Biology

Texto completo disponível

Citações Citado por
  • Título:
    Vasopressin-dependent Inhibition of the C-type Natriuretic Peptide Receptor, NPR-B/GC-B, Requires Elevated Intracellular Calcium Concentrations
  • Autor: Abbey, Sarah E ; Potter, Lincoln R
  • Assuntos: Animals ; Aorta - physiology ; Arginine Vasopressin - pharmacology ; Atrial Natriuretic Factor - pharmacology ; Calcium - physiology ; Cell Membrane - physiology ; Cells, Cultured ; Cyclic AMP - metabolism ; Guanylate Cyclase - drug effects ; Guanylate Cyclase - metabolism ; Kinetics ; Muscle, Smooth, Vascular - physiology ; Natriuretic Peptide, C-Type - pharmacology ; Rats ; Receptors, Atrial Natriuretic Factor - drug effects ; Receptors, Atrial Natriuretic Factor - metabolism ; Tetradecanoylphorbol Acetate - pharmacology ; Vasopressins - pharmacology
  • É parte de: The Journal of biological chemistry, 2002-11, Vol.277 (45), p.42423-42430
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-1
    content type line 23
  • Descrição: Natriuretic peptides bind their cognate cell surface guanylyl cyclase receptors and elevate intracellular cGMP concentrations. In vascular smooth muscle cells, this results in the activation of the type I cGMP-dependent protein kinase and vasorelaxation. In contrast, pressor hormones like arginine-vasopressin, angiotensin II, and endothelin bind serpentine receptors that interact with G q and activate phospholipase Cβ. The products of this enzyme, diacylglycerol and inositol trisphosphate, activate the conventional and novel forms of protein kinase C (PKC) and elevate intracellular calcium concentrations, respectively. The latter response results in vasoconstriction, which opposes the actions of natriuretic peptides. Previous reports have shown that pressor hormones inhibit natriuretic peptide receptors NPR-A or NPR-B in a variety of different cell types. Although the mechanism for this inhibition remains unknown, it has been universally accepted that PKC is an obligatory component of this pathway primarily because pharmacologic activators of PKC mimic the inhibitory effects of these hormones. Here, we show that in A10 vascular smooth muscle cells, neither chronic PKC down-regulation nor specific PKC inhibitors block the AVP-dependent desensitization of NPR-B even though both processes block PKC-dependent desensitization. In contrast, the cell-permeable calcium chelator, BAPTA-AM (1,2-bis(2-aminophenoxy)ethane- N,N,N ′, N ′-tetraacetic acid, tetraacetoxymethyl ester), abrogates the AVP-dependent desensitization of NPR-B, and ionomycin, a calcium ionophore, mimics the AVP effect. These data show that the inositol trisphosphate/calcium arm of the phospholipase C pathway mediates the desensitization of a natriuretic peptide receptor in A10 cells. In addition, we report that CNP attenuates AVP-dependent elevations in intracellular calcium concentrations. Together, these data reveal a dominant role for intracellular calcium in the reciprocal regulation of these two important vasoactive signaling systems.
  • Editor: United States: American Society for Biochemistry and Molecular Biology
  • Idioma: Inglês
Links
Voltar para lista de resultados
Resultado  1 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.