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Identification of newly established Spodoptera littoralis cell lines by two DNA barcoding markers

Ahmed, I. ; Huebner, H. ; Mamoori, Y. I. ; Buchholz, R.

In vitro cellular & developmental biology. Animal, 2017-04, Vol.53 (4), p.288-292 [Periódico revisado por pares]

New York: Springer Science & Business Media LLC

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Título:
Identification of newly established Spodoptera littoralis cell lines by two DNA barcoding markers
Autor: Ahmed, I. ; Huebner, H. ; Mamoori, Y. I. ; Buchholz, R.
Assuntos: Amplification ; Animal Genetics and Genomics ; Animals ; Base Sequence ; Biology ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Cell Culture ; Cell Line - cytology ; Cell lines ; Contamination ; Cytochrome ; Cytochrome-c oxidase ; Cytochromes ; Deoxyribonucleic acid ; Developmental Biology ; DNA ; DNA barcoding ; DNA Barcoding, Taxonomic ; DNA fingerprinting ; Electron Transport Complex IV - genetics ; Electrophoresis, Agar Gel ; Gene sequencing ; Genes ; Genetic fingerprinting ; Genetic Markers ; Genomics ; Identification ; Insects ; Laboratories ; Life Sciences ; Lymphocytes B ; Mitochondria ; Mitochondrial DNA ; Organisms ; Phylogenetics ; Phylogeny ; Primers ; Sequence Alignment ; Similarity ; Spodoptera - cytology ; Spodoptera - genetics ; Spodoptera littoralis ; Stem Cells ; Vaccines
É parte de: In vitro cellular & developmental biology. Animal, 2017-04, Vol.53 (4), p.288-292
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Cell line authentication is crucial in determining the identity of cell lines and detecting any cross-contamination. The identity of three newly established Spodoptera littoralis cell lines (Spli-C, Spli-B, and Spli-S) was confirmed by DNA fingerprinting. In this study, we used two universal primers sets to amplify two DNA fragments in different positions in the mitochondrial cytochrome C oxidase 1 gene (COI). The PCR reaction succeeded in amplifying two target DNA amplicons. The first amplicon had ∼650 bp, while the second had ∼410 bp. By comparing the obtained informative sequences with those in the GenBank sequence database, the results showed 100% similarity between the S. littoralis cell lines and their host. The same similarity ratio was observed between the Sf21, Tni, and Cp cell lines, which are used widely, and their hosts. The informative sequences were then used for phylogenetic analyses. In addition to the high efficiency of this technique, it showed high reproducibility in two different laboratories. DNA barcoding using the two sets of the universal primers used in this study can be a fast and a reliable method for insect cell line identification.
Editor: New York: Springer Science & Business Media LLC
Idioma: Inglês

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Identification of newly established Spodoptera littoralis cell lines by two DNA barcoding markers

Ahmed, I. ; Huebner, H. ; Mamoori, Y. I. ; Buchholz, R.

New York: Springer Science & Business Media LLC

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