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Therapeutic treatment with scFv–PLGA nanoparticles decreases pulmonary fungal load in a murine model of paracoccidioidomycosis

Jannuzzi, Grasielle Pereira ; Souza, Nicole de Araújo ; Françoso, Kátia Sanches ; Pereira, Roney Henrique ; Santos, Raquel Possemozer ; Kaihami, Gilberto Hideo ; Almeida, José Roberto Fogaça de ; Batista, Wagner Luiz ; Amaral, André Corrêa ; Maranhão, Andrea Queiroz ; Almeida, Sandro Rogério de ; Ferreira, Karen Spadari

Microbes and infection, 2018-01, Vol.20 (1), p.48-56 [Periódico revisado por pares]

France: Elsevier Masson SAS

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  • Título:
    Therapeutic treatment with scFv–PLGA nanoparticles decreases pulmonary fungal load in a murine model of paracoccidioidomycosis
  • Autor: Jannuzzi, Grasielle Pereira ; Souza, Nicole de Araújo ; Françoso, Kátia Sanches ; Pereira, Roney Henrique ; Santos, Raquel Possemozer ; Kaihami, Gilberto Hideo ; Almeida, José Roberto Fogaça de ; Batista, Wagner Luiz ; Amaral, André Corrêa ; Maranhão, Andrea Queiroz ; Almeida, Sandro Rogério de ; Ferreira, Karen Spadari
  • Assuntos: Paracoccidioides brasiliensis ; Paracoccidioidomycosis ; PLGA ; scFv
  • É parte de: Microbes and infection, 2018-01, Vol.20 (1), p.48-56
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Paracoccidioidomycosis (PCM) is a systemic mycosis with lymphatic dissemination that is caused by Paracoccidioides species. Treatment of PCM consists of chemotherapeutics such as itraconazole, trimethoprim, sulfamethoxazole or amphotericin B. However, several studies are aiming to develop therapeutic alternatives for the treatment of fungal infection using new molecules as adjuvants. The single-chain variable fragments (scFv) from an antibody that mimics the main fungal component incorporated within poly(lactide-co-glycolic) acid (PLGA) nanoparticles helped treat the fungal disease. After expressing the scFv in Picchia pastoris (P. pastoris), the recombinant molecules were coupled with PLGA, and the BALB/c mice were immunized before or after infection with yeast Paracoccidioides brasiliensis (P. brasiliensis). Our results showed decreased disease progression and decreased fungal burden. Taken together, our results showed an increased of IFN-γ and IL-12 cytokine production and an increased number of macrophages and dendritic cells in the pulmonary tissue of BALB/c mice treated with a high concentration of our molecule. Our data further confirm that the scFv plays an important role in the treatment of experimental PCM.
  • Editor: France: Elsevier Masson SAS
  • Idioma: Inglês
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