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von Willebrand factor propeptide to antigen ratio identifies platelet activation and reduced von Willebrand factor survival phenotype in mice

Jacobi, P. M. ; Kanaji, S. ; Jakab, D. ; Gehrand, A. L. ; Johnsen, J. M. ; Haberichter, S. L.

Journal of thrombosis and haemostasis, 2018-03, Vol.16 (3), p.546-554 [Periódico revisado por pares]

England: Wiley Subscription Services, Inc

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Título:
von Willebrand factor propeptide to antigen ratio identifies platelet activation and reduced von Willebrand factor survival phenotype in mice
Autor: Jacobi, P. M. ; Kanaji, S. ; Jakab, D. ; Gehrand, A. L. ; Johnsen, J. M. ; Haberichter, S. L.
Assuntos: Alleles ; Animals ; Antigens ; Antigens - chemistry ; Blood Coagulation ; Blood platelets ; Blood Platelets - cytology ; Bone Marrow Transplantation ; Disease Models, Animal ; Endothelium ; Genotype & phenotype ; HEK293 Cells ; Hemorrhage ; Humans ; Mice ; Mice, Inbred C57BL ; Peptides - chemistry ; Phenotype ; Phenotypes ; Phlebotomy ; Platelet Activation ; Platelets ; Protein Precursors - blood ; Reproducibility of Results ; Sampling ; Saphenous Vein ; Survival ; von Willebrand disease ; von Willebrand Diseases - genetics ; Von Willebrand factor ; von Willebrand Factor - chemistry ; von willebrand factor propolypeptide
É parte de: Journal of thrombosis and haemostasis, 2018-03, Vol.16 (3), p.546-554
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Essentials Reduced survival of von Willebrand factor (VWF) in plasma causes type 1C von Willebrand disease. Blood was collected from mouse strains by various methods and VWF propeptide and antigen assayed. VWF propeptide to antigen ratio identifies a reduced VWF survival phenotype in mice. This ratio validates the acceptability of murine blood samples for coagulation studies. Summary Background Reduced plasma survival of von Willebrand factor (VWF) is characteristic of patients with type 1C von Willebrand disease (VWD). These subjects can be identified by an increased steady‐state ratio of plasma VWF propeptide (VWFpp) to VWF antigen (VWF:Ag). A similar phenotype occurs in mice with the Mvwf1 allele. Objectives To (i) determine if the VWFpp/VWF:Ag ratio can be used to identify a ‘type 1C’ phenotype in mice, (ii) determine the most reliable method for murine blood sampling, and (iii) identify the source of VWF released during problematic blood collection. Methods ‘Platelet‐VWF’ and ‘endothelial‐VWF’ mice were generated by bone marrow transplantation between C57BL/6J and VWF‐/‐ mice. Several blood sampling methods were used and murine VWFpp and VWF:Ag levels determined. Plasma and platelet VWF:Ag and VWFpp, VWF multimers and VWF half‐life were examined in mouse strains with and without Mvwf1. Results A single retro‐orbital bleed and vena cava collection were found to be the optimal methods of blood collection. Problematic collection resulted in release of VWF from platelets and endothelium. The VWFpp/VWF:Ag ratio identified strains of mice with reduced VWF survival. Conclusion Assay of murine VWFpp and VWF:Ag has utility in determining the acceptability of murine blood samples for coagulation testing and in identification of a reduced VWF survival phenotype in mice.
Editor: England: Wiley Subscription Services, Inc
Idioma: Inglês

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von Willebrand factor propeptide to antigen ratio identifies platelet activation and reduced von Willebrand factor survival phenotype in mice

Jacobi, P. M. ; Kanaji, S. ; Jakab, D. ; Gehrand, A. L. ; Johnsen, J. M. ; Haberichter, S. L.

England: Wiley Subscription Services, Inc

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