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Shifts in gene expression profiles are associated with weak and strong Crassulacean acid metabolism

Heyduk, Karolina ; Ray, Jeremy N. ; Ayyampalayam, Saravanaraj ; Leebens-Mack, James

American journal of botany, 2018-03, Vol.105 (3), p.587-601 [Periódico revisado por pares]

United States: John Wiley and Sons, Inc

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Título:
Shifts in gene expression profiles are associated with weak and strong Crassulacean acid metabolism
Autor: Heyduk, Karolina ; Ray, Jeremy N. ; Ayyampalayam, Saravanaraj ; Leebens-Mack, James
Assuntos: Abiotic factors ; Acidity ; Adaptation, Biological ; Agave ; Agavoideae ; Asparagaceae - genetics ; Asparagaceae - metabolism ; Biofuels ; Biological Evolution ; Carbohydrate metabolism ; Carbohydrate Metabolism - genetics ; Carbohydrates ; Carbon - metabolism ; Climate ; Crassulacean acid metabolism ; Gas exchange ; gene coexpression ; Gene Expression ; Gene sequencing ; Genes ; Genes, Plant ; Genome, Plant ; Genomics - methods ; High-Throughput Nucleotide Sequencing ; Homology ; INVITED SPECIAL ARTICLE: For the Special Issue: Using and Navigating the Plant Tree of Life ; Metabolism ; Network analysis ; Next-generation sequencing ; Phenotype ; Phenotypes ; Photosynthesis ; Photosynthesis - genetics ; Phylogenetics ; Phylogeny ; Ribonucleic acid ; RNA ; RNA, Plant - analysis ; RNA‐seq ; RNA‐sequencing ; Sequence Analysis, RNA ; Species ; Species Specificity ; Sugar ; Sugars - metabolism ; Transcriptome ; transcriptomics ; weak CCAM
É parte de: American journal of botany, 2018-03, Vol.105 (3), p.587-601
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Descrição: Premise of the Study The relative ease of high throughput sequencing is facilitating comprehensive phylogenomic and gene expression studies, even for nonmodel groups. To date, however, these two approaches have not been merged; while phylogenomic methods might use transcriptome sequences to resolve relationships, assessment of gene expression patterns in a phylogenetic context is less common. Here we analyzed both carbon assimilation and gene expression patterns of closely related species within the Agavoideae (Asparagaceae) to elucidate changes in gene expression across weak and strong phenotypes for Crassulacean acid metabolism (CAM). Methods Gene expression patterns were compared across four genera: Agave (CAM), which is paraphyletic with Polianthes (weak CAM) and Manfreda (CAM), and Beschorneria (weak CAM). RNA‐sequencing was paired with measures of gas exchange and titratable acidity. Climate niche space was compared across the four lineages to examine abiotic factors and their correlation to CAM. Key Results Expression of homologous genes showed both shared and variable patterns in weak and strong CAM species. Network analysis highlights that despite shared expression patterns, highly connected genes differ between weak and strong CAM, implicating shifts in regulatory gene function as key for the evolution of CAM. Variation in carbohydrate metabolism between weak and strong CAM supports the importance of sugar turnovers for CAM physiology. Conclusions Integration of phylogenetics and RNA‐sequencing provides a powerful tool to study the evolution of CAM photosynthesis across closely related but photosynthetically variable species. Our findings regarding shared or shifted gene expression and regulation of CAM via carbohydrate metabolism have important implications for efforts to engineer the CAM pathway into C3 food and biofuel crops.
Editor: United States: John Wiley and Sons, Inc
Idioma: Inglês

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Shifts in gene expression profiles are associated with weak and strong Crassulacean acid metabolism

Heyduk, Karolina ; Ray, Jeremy N. ; Ayyampalayam, Saravanaraj ; Leebens-Mack, James

United States: John Wiley and Sons, Inc

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