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Microscopy-Based Chromosome Conformation Capture Enables Simultaneous Visualization of Genome Organization and Transcription in Intact Organisms

Cardozo Gizzi, Andrés M. ; Cattoni, Diego I. ; Fiche, Jean-Bernard ; Espinola, Sergio M. ; Gurgo, Julian ; Messina, Olivier ; Houbron, Christophe ; Ogiyama, Yuki ; Papadopoulos, Giorgio L. ; Cavalli, Giacomo ; Lagha, Mounia ; Nollmann, Marcelo

Molecular cell, 2019-04, Vol.74 (1), p.212-222.e5 [Peer Reviewed Journal]

United States: Elsevier Inc

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Title:
Microscopy-Based Chromosome Conformation Capture Enables Simultaneous Visualization of Genome Organization and Transcription in Intact Organisms
Author: Cardozo Gizzi, Andrés M. ; Cattoni, Diego I. ; Fiche, Jean-Bernard ; Espinola, Sergio M. ; Gurgo, Julian ; Messina, Olivier ; Houbron, Christophe ; Ogiyama, Yuki ; Papadopoulos, Giorgio L. ; Cavalli, Giacomo ; Lagha, Mounia ; Nollmann, Marcelo
Subjects: Animals ; Cell Cycle - genetics ; chromatin ; Chromatin - genetics ; Chromatin - metabolism ; Chromatin Assembly and Disassembly ; chromosome conformation capture ; Chromosomes, Insect - genetics ; Drosophila development ; Drosophila melanogaster - embryology ; Drosophila melanogaster - genetics ; Drosophila melanogaster - metabolism ; fluorescence microscopy ; fluorescent in situ hybridization ; Gene Expression Regulation, Developmental ; Genome ; genome architecture ; genome organization ; High-Throughput Nucleotide Sequencing - methods ; In Situ Hybridization, Fluorescence ; Microscopy, Fluorescence - methods ; oligopaint ; RNA - biosynthesis ; RNA - genetics ; Single-Cell Analysis - methods ; topologically associating domains ; Transcription, Genetic ; Transcriptional Activation ; transcriptional regulation
Is Part Of: Molecular cell, 2019-04, Vol.74 (1), p.212-222.e5
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: Eukaryotic chromosomes are organized in multiple scales, from nucleosomes to chromosome territories. Recently, genome-wide methods identified an intermediate level of chromosome organization, topologically associating domains (TADs), that play key roles in transcriptional regulation. However, these methods cannot directly examine the interplay between transcriptional activation and chromosome architecture while maintaining spatial information. Here we present a multiplexed, sequential imaging approach (Hi-M) that permits simultaneous detection of chromosome organization and transcription in single nuclei. This allowed us to unveil the changes in 3D chromatin organization occurring upon transcriptional activation and homologous chromosome unpairing during awakening of the zygotic genome in intact Drosophila embryos. Excitingly, the ability of Hi-M to explore the multi-scale chromosome architecture with spatial resolution at different stages of development or during the cell cycle will be key to understanding the mechanisms and consequences of the 4D organization of the genome. [Display omitted] •Hi-M simultaneously reveals 3D chromatin organization and transcriptional activity•Hi-M and Hi-C maps agree across several orders of magnitude•Chromatin is spatially compacted into TADs after the midblastula transition•TAD internal organization dramatically changes upon transcriptional activation Cardozo Gizzi et al. developed Hi-M, a multiplexed imaging-based approach to detect 3D chromatin folding in single cells within intact Drosophila embryos. The ability of Hi-M to detect the spatial organization of cells enabled measurement of changes in TAD organization during early embryogenesis and upon transcriptional activation.
Publisher: United States: Elsevier Inc
Language: English

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Microscopy-Based Chromosome Conformation Capture Enables Simultaneous Visualization of Genome Organization and Transcription in Intact Organisms

Cardozo Gizzi, Andrés M. ; Cattoni, Diego I. ; Fiche, Jean-Bernard ; Espinola, Sergio M. ; Gurgo, Julian ; Messina, Olivier ; Houbron, Christophe ; Ogiyama, Yuki ; Papadopoulos, Giorgio L. ; Cavalli, Giacomo ; Lagha, Mounia ; Nollmann, Marcelo

United States: Elsevier Inc

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