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Visual Quantitative Detection of Circulating Tumor Cells with Single‐Cell Sensitivity Using a Portable Microfluidic Device

Abate, Mahlet Fasil ; Jia, Shasha ; Ahmed, Metages Gashaw ; Li, Xingrui ; Lin, Li ; Chen, Xiaoqian ; Zhu, Zhi ; Yang, Chaoyong

Small (Weinheim an der Bergstrasse, Germany), 2019-04, Vol.15 (14), p.e1804890-n/a [Peer Reviewed Journal]

Germany: Wiley Subscription Services, Inc

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  • Title:
    Visual Quantitative Detection of Circulating Tumor Cells with Single‐Cell Sensitivity Using a Portable Microfluidic Device
  • Author: Abate, Mahlet Fasil ; Jia, Shasha ; Ahmed, Metages Gashaw ; Li, Xingrui ; Lin, Li ; Chen, Xiaoqian ; Zhu, Zhi ; Yang, Chaoyong
  • Subjects: Blood circulation ; Catalysis ; circulating tumor cells ; Hydrogen peroxide ; Leukocytes ; Microfluidic devices ; microfluidics ; Nanoparticles ; Nanotechnology ; portable device ; Portable equipment ; Sensitivity ; single‐cell sensitivity ; Target detection ; Tumors ; visual quantification
  • Is Part Of: Small (Weinheim an der Bergstrasse, Germany), 2019-04, Vol.15 (14), p.e1804890-n/a
  • Notes: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Description: Immunocytological technologies, molecular technologies, and functional assays are widely used for detecting circulating tumor cells (CTCs) after enrichment from patients' blood sample. Unfortunately, accessibility to these technologies is limited due to the need for sophisticated instrumentation and skilled operators. Portable microfluidic devices have become attractive tools for expanding the access and efficiency of detection beyond hospitals to sites near the patient. Herein, a volumetric bar chart chip (V‐Chip) is developed as a portable platform for CTC detection. The target CTCs are labeled with aptamer‐conjugated nanoparticles (ACNPs) and analyzed by V‐Chip through quantifying the byproduct (oxygen) of the catalytic reaction between ACNPs and hydrogen peroxide, which results in the movement of an ink bar to a concentration‐dependent distance for visual quantitative readout. Thus, the CTC number is decoded into visually quantifiable information and a linear correlation can be found between the distance moved by the ink and number of cells in the sample. This method is sensitive enough that a single cell can be detected. Furthermore, the clinical capabilities of this system are demonstrated for quantitative CTC detection in the presence of a high leukocyte background. This portable detection method shows great potential for quantification of rare cells with single‐cell sensitivity for various applications. Visual quantitative detection of circulating tumor cells (CTCs) is realized by using a portable volumetric bar chart chip (V‐Chip) with single‐cell sensitivity. The target CTCs are labeled with aptamer‐conjugated nanoparticles that can catalyze the generation of oxygen for the movement of an ink bar in the V‐Chip. The CTC number can be quantified visually by this movement.
  • Publisher: Germany: Wiley Subscription Services, Inc
  • Language: English
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