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Seeing is believing? When scanning electron microscopy (SEM) meets clinical dentistry: The replica technique

Naves, Lucas Zago ; Gerdolle, David‐Alain ; Andrade, Oswaldo Scopin ; Gresnigt, Marco

Microscopy research and technique, 2020-09, Vol.83 (9), p.1118-1123 [Periódico revisado por pares]

Hoboken, USA: John Wiley & Sons, Inc

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  • Título:
    Seeing is believing? When scanning electron microscopy (SEM) meets clinical dentistry: The replica technique
  • Autor: Naves, Lucas Zago ; Gerdolle, David‐Alain ; Andrade, Oswaldo Scopin ; Gresnigt, Marco
  • Assuntos: Adult ; Chipping ; clinical dentistry ; Dental Enamel - ultrastructure ; Dental materials ; Dentin - ultrastructure ; Dentistry ; Dentistry - methods ; Female ; Fracture mechanics ; Humans ; in vivo evaluation ; Interfaces ; magnification ; Microscopy, Electron, Scanning ; Middle Aged ; replica technique ; Replica Techniques - methods ; Replicating ; Scanning electron microscopy ; Surface properties ; Tooth - ultrastructure ; Workflow
  • É parte de: Microscopy research and technique, 2020-09, Vol.83 (9), p.1118-1123
  • Notas: Paul Verkade
    Review Editor
    ObjectType-Case Study-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-4
    content type line 23
    ObjectType-Report-1
    ObjectType-Article-3
  • Descrição: In restorative dentistry, the in situ replication of intra‐oral situations, is based on a non‐invasive and non‐destructive scanning electron microscopy (SEM) evaluation method. The technique is suitable for investigation restorative materials and dental hard‐ and soft‐tissues, and its interfaces. Surface characteristics, integrity of interfaces (margins), or fracture analysis (chipping, cracks, etc.) with reliable resolution and under high magnification (from ×50 to ×5,000). Overall the current study aims to share detailed and reproducible information about the replica technique. Specific goals are: (a) to describe detailed each step involved in producing a replica of an intra‐oral situation, (b) to validate an integrated workflow based on a rational sequence from visual examination, to macrophotography and SEM analysis using the replica technique; (c) to present three clinical cases documented using the technique. A compilation of three clinical situations/cases were analyzed here by means the replica technique showing a wide range of possibilities that can be reached and explored with the described technique. This guidance document will contribute to a more accurate use of the replica technique and help researchers and clinicians to understand and identify issues related to restorative procedures under high magnification. The replica technique figures as a versatile approach to use scanning electron microscopy (SEM) as research tool to analyze and follow‐up intra oral situations; The current study aims to describe, in a detailed step by step, the specimens confection—from the intra‐oral impression procedures to SEM micrograph interpretation; Despite technical sensitivity, the SEM/replica technique could be considered a reference standard for high magnification clinical evaluation, and has a potential for follow‐up studies by means of specific comparisons over the time.
  • Editor: Hoboken, USA: John Wiley & Sons, Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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