Idioma:

BANP opens chromatin and activates CpG-island-regulated genes

Grand, Ralph S ; Burger, Lukas ; Gräwe, Cathrin ; Michael, Alicia K ; Isbel, Luke ; Hess, Daniel ; Hoerner, Leslie ; Iesmantavicius, Vytautas ; Durdu, Sevi ; Pregnolato, Marco ; Krebs, Arnaud R ; Smallwood, Sébastien A ; Thomä, Nicolas ; Vermeulen, Michiel ; Schübeler, Dirk

Nature (London), 2021-08, Vol.596 (7870), p.133-137 [Periódico revisado por pares]

England: Nature Publishing Group

Texto completo disponível

Citações Citado por

Enviar para

Título:
BANP opens chromatin and activates CpG-island-regulated genes
Autor: Grand, Ralph S ; Burger, Lukas ; Gräwe, Cathrin ; Michael, Alicia K ; Isbel, Luke ; Hess, Daniel ; Hoerner, Leslie ; Iesmantavicius, Vytautas ; Durdu, Sevi ; Pregnolato, Marco ; Krebs, Arnaud R ; Smallwood, Sébastien A ; Thomä, Nicolas ; Vermeulen, Michiel ; Schübeler, Dirk
Assuntos: Animals ; Base Sequence ; Binders ; Binding sites ; Cancer ; Cell cycle ; Cell Cycle Proteins - metabolism ; Cell differentiation ; Cell Line, Tumor ; Chromatin ; Chromatin - chemistry ; Chromatin - genetics ; Chromatin - metabolism ; Chromatin Assembly and Disassembly - genetics ; CpG islands ; CpG Islands - genetics ; Deoxyribonucleic acid ; DNA ; DNA Methylation ; DNA-Binding Proteins - metabolism ; DNA-directed RNA polymerase ; Epigenetics ; Footprinting ; Gene Expression Regulation ; Gene loci ; Genes ; Genes, Essential ; Genomes ; Human tissues ; Humans ; Metabolism ; Mice ; Nuclear Proteins - metabolism ; Nucleosomes ; Pluripotency ; Proteins ; Proteomics ; RNA polymerase ; RNA polymerase II ; Single Molecule Imaging ; Transcription factors
É parte de: Nature (London), 2021-08, Vol.596 (7870), p.133-137
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The majority of gene transcripts generated by RNA polymerase II in mammalian genomes initiate at CpG island (CGI) promoters , yet our understanding of their regulation remains limited. This is in part due to the incomplete information that we have on transcription factors, their DNA-binding motifs and which genomic binding sites are functional in any given cell type . In addition, there are orphan motifs without known binders, such as the CGCG element, which is associated with highly expressed genes across human tissues and enriched near the transcription start site of a subset of CGI promoters . Here we combine single-molecule footprinting with interaction proteomics to identify BTG3-associated nuclear protein (BANP) as the transcription factor that binds this element in the mouse and human genome. We show that BANP is a strong CGI activator that controls essential metabolic genes in pluripotent stem and terminally differentiated neuronal cells. BANP binding is repelled by DNA methylation of its motif in vitro and in vivo, which epigenetically restricts most binding to CGIs and accounts for differential binding at aberrantly methylated CGI promoters in cancer cells. Upon binding to an unmethylated motif, BANP opens chromatin and phases nucleosomes. These findings establish BANP as a critical activator of a set of essential genes and suggest a model in which the activity of CGI promoters relies on methylation-sensitive transcription factors that are capable of chromatin opening.
Editor: England: Nature Publishing Group
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 4 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

BANP opens chromatin and activates CpG-island-regulated genes

Grand, Ralph S ; Burger, Lukas ; Gräwe, Cathrin ; Michael, Alicia K ; Isbel, Luke ; Hess, Daniel ; Hoerner, Leslie ; Iesmantavicius, Vytautas ; Durdu, Sevi ; Pregnolato, Marco ; Krebs, Arnaud R ; Smallwood, Sébastien A ; Thomä, Nicolas ; Vermeulen, Michiel ; Schübeler, Dirk

England: Nature Publishing Group

Buscando em bases de dados remotas. Favor aguardar.