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In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes

Shirosaki, Yuki ; Tsuru, Kanji ; Hayakawa, Satoshi ; Osaka, Akiyoshi ; Lopes, Maria Ascensão ; Santos, José Domingos ; Fernandes, Maria Helena

Biomaterials, 2005-02, Vol.26 (5), p.485-493 [Periódico revisado por pares]

Netherlands: Elsevier Ltd

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Título:
In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes
Autor: Shirosaki, Yuki ; Tsuru, Kanji ; Hayakawa, Satoshi ; Osaka, Akiyoshi ; Lopes, Maria Ascensão ; Santos, José Domingos ; Fernandes, Maria Helena
Assuntos: Alkaline Phosphatase - analysis ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Biocompatible Materials - toxicity ; Bone Neoplasms - pathology ; Calcium - pharmacology ; Cell Adhesion - drug effects ; Cell Differentiation - drug effects ; Cell Division - drug effects ; Cell Line, Tumor - cytology ; Cell Line, Tumor - drug effects ; Chitosan ; Chitosan - chemistry ; Chitosan - pharmacology ; Chitosan - toxicity ; Cross-Linking Reagents - pharmacology ; Cytocompatibility ; Humans ; Magnetic Resonance Spectroscopy ; Materials Testing ; Membranes, Artificial ; MG63 osteoblastic cells ; Microscopy, Electron, Scanning ; Neoplasm Proteins - analysis ; Organic–inorganic hybrid ; Osteoblasts - cytology ; Osteoblasts - drug effects ; Osteoblasts - enzymology ; Osteosarcoma - pathology ; Silanes - pharmacology ; Spectroscopy, Fourier Transform Infrared ; Wettability ; γ-Glycidoxypropyltrimethoxysilane (GPSM)
É parte de: Biomaterials, 2005-02, Vol.26 (5), p.485-493
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Descrição: Chitosan-silicate hybrids were synthesized using γ-glycidoxypropyltrimethoxysilane (GPSM) as the agent for cross-linking the chitosan chains. CaCl 2 was introduced in the hybrids in expectation that it would improve cell adhesion and differentiation of the hybrid surfaces. Fourier-transform infrared (FT–IR) spectroscopy and 29Si CP-MAS NMR spectroscopy were used to analyze the structures of the hybrids. Cytocompatibility of the hybrids was investigated in terms of proliferation of an osteoblastic cell line, MG63. The adhesion and proliferation of the osteoblastic cells cultured on the surface of a chitosan-GPSM hybrid without calcium were similar to those on a control culture plate, and were better than those on a chitosan membrane. The ALP activity of the cells cultured on this hybrid was higher than that on the chitosan membrane. Contrary to expectations, the incorporation of calcium ions into the hybrids did not improve cell attachment and proliferation on their surfaces.
Editor: Netherlands: Elsevier Ltd
Idioma: Inglês

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In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes

Shirosaki, Yuki ; Tsuru, Kanji ; Hayakawa, Satoshi ; Osaka, Akiyoshi ; Lopes, Maria Ascensão ; Santos, José Domingos ; Fernandes, Maria Helena

Netherlands: Elsevier Ltd

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