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Complementation of replication origin function in mouse embryonic stem cells by human DNA sequences

Cohen, Stephanie M. ; Hatada, Seigo ; Brylawski, Bruna P. ; Smithies, Oliver ; Kaufman, David G. ; Cordeiro-Stone, Marila

Genomics (San Diego, Calif.), 2004-09, Vol.84 (3), p.475-484 [Periódico revisado por pares]

San Diego, CA: Elsevier Inc

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Título:
Complementation of replication origin function in mouse embryonic stem cells by human DNA sequences
Autor: Cohen, Stephanie M. ; Hatada, Seigo ; Brylawski, Bruna P. ; Smithies, Oliver ; Kaufman, David G. ; Cordeiro-Stone, Marila
Assuntos: Animals ; Base Sequence ; Biological and medical sciences ; DNA Primers ; Functional homology ; Fundamental and applied biological sciences. Psychology ; Gene Components ; Genes. Genome ; Genetics of eukaryotes. Biological and molecular evolution ; HPRT ; Humans ; Hypoxanthine Phosphoribosyltransferase - genetics ; Mice - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Polymerase Chain Reaction ; Quantitative PCR ; Recombination, Genetic - genetics ; Replication origin ; Replication Origin - genetics ; Sequence Alignment ; Stem Cells
É parte de: Genomics (San Diego, Calif.), 2004-09, Vol.84 (3), p.475-484
Notas: ObjectType-Article-2
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Descrição: A functional origin of replication was mapped to the transcriptional promoter and exon 1 of the hypoxanthine–guanine phosphoribosyltransferase (HPRT) gene in the mouse and human genomes. This origin was lost in mouse embryonic stem (ES) cells with a spontaneous deletion (∼36 kb) at the 5′ end of the HPRT locus. Restoration of HPRT activity by homologous recombination with human/mouse chimeric sequences reconstituted replication origin activity in two independent ES cell lines. Quantitative PCR analyses of abundance of genetic markers in size-fractionated nascent DNA indicated that initiation of DNA replication coincided with the site of insertion in the mouse genome of the 335 bp of human DNA containing the HPRT exon 1 and a truncated promoter. The genetic information contained in the human sequence and surrounding mouse DNA was analyzed for cis-acting elements that might contribute to selection and functional activation of a mammalian origin of DNA replication.
Editor: San Diego, CA: Elsevier Inc
Idioma: Inglês

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Complementation of replication origin function in mouse embryonic stem cells by human DNA sequences

Cohen, Stephanie M. ; Hatada, Seigo ; Brylawski, Bruna P. ; Smithies, Oliver ; Kaufman, David G. ; Cordeiro-Stone, Marila

San Diego, CA: Elsevier Inc

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