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Adenosine receptors involved in modulation of noradrenaline release in isolated rat tail artery

Diniz, Carmen ; Fresco, Paula ; Leal, Sandra ; Gonçalves, Jorge

European journal of pharmacology, 2004-11, Vol.504 (1), p.17-25 [Periódico revisado por pares]

Amsterdam: Elsevier B.V

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Título:
Adenosine receptors involved in modulation of noradrenaline release in isolated rat tail artery
Autor: Diniz, Carmen ; Fresco, Paula ; Leal, Sandra ; Gonçalves, Jorge
Assuntos: Adenosine A 2A receptor ; Adenosine A 2B receptor ; Adenosine-5'-(N-ethylcarboxamide) - pharmacology ; Animals ; Arteries ; Biological and medical sciences ; Dose-Response Relationship, Drug ; Immunohistochemistry ; Male ; Medical sciences ; Noradrenaline release ; Norepinephrine - blood ; Norepinephrine - secretion ; Pharmacology. Drug treatments ; Prejunctional adenosine receptor ; Purinergic P1 Receptor Agonists ; Purinergic P1 Receptor Antagonists ; Pyrimidines - pharmacology ; rat ; Rats ; Receptors, Purinergic P1 - physiology ; Tail - blood supply ; Tail - drug effects ; Tail - secretion ; Tail artery ; Triazoles - pharmacology
É parte de: European journal of pharmacology, 2004-11, Vol.504 (1), p.17-25
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Adenosine receptors involved in the modulation of noradrenaline release from postganglionic sympathetic nerves in rat tail artery were characterized by studying the effects of adenosine-receptor agonists and antagonists on electrically evoked tritium overflow (100 pulses, 5 Hz) and by immunohistochemistry. The adenosine A 1 receptor-selective agonist N 6-cyclopentyladenosine (CPA; 1–100 nM) and the non-selective adenosine receptor agonist N-ethylcarboxamidoadenosine (NECA; 1–10 μM) decreased tritium overflow. These effects were blocked by the adenosine A 1 receptor-selective antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX; 30 nM). The adenosine A 2A receptor-selective agonist 2- p–(2-carboxyethyl)phenethylamino-5′- N-ethylcarboxamido adenosine (CGS 21680; 1–100 nM) enhanced tritium overflow, an effect blocked by the adenosine A 2A receptor-selective antagonist 5-amino-7-(2-phenylethyl)-2-(2-furyl)-pyrazolo-[4,3- e]-1,2,4-triazolo[1,5- c]pyrimidine (SCH 58261; 20 nM) but not changed by the adenosine A 2B receptor-selective antagonist N-(4-acetylphenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3-dipropyl-1 H-purin-8-yl) phenoxy]acetamide (MRS 1706; 20 nM). In the presence of DPCPX (30 nM), NECA enhanced tritium overflow, an effect abolished by MRS 1706 but not influenced by SCH 58261. Immunohistochemistry revealed immunoreactivity for all adenosine-receptor subtypes. Areas of co-localization were found for neurofilament with adenosine A 1, A 2A and A 2B but not A 3 receptors. In conclusion, the present study provides functional and morphological evidence for the occurrence of multiple adenosine receptor-mediated modulation of noradrenaline release in the rat tail: inhibition mediated by adenosine A 1 receptors and facilitation mediated by both adenosine A 2A and A 2B receptors.
Editor: Amsterdam: Elsevier B.V
Idioma: Inglês

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Adenosine receptors involved in modulation of noradrenaline release in isolated rat tail artery

Diniz, Carmen ; Fresco, Paula ; Leal, Sandra ; Gonçalves, Jorge

Amsterdam: Elsevier B.V

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