Idioma:

Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection

Kobae, Y.(Nagoya Univ. (Japan)) ; Sekino, T ; Yoshioka, H ; Nakagawa, T ; Martinoia, E ; Maeshima, M

Plant and cell physiology, 2006-03, Vol.47 (3), p.309-318 [Periódico revisado por pares]

Japan: Oxford University Press

Texto completo disponível

Citações Citado por

Enviar para

Título:
Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection
Autor: Kobae, Y.(Nagoya Univ. (Japan)) ; Sekino, T ; Yoshioka, H ; Nakagawa, T ; Martinoia, E ; Maeshima, M
Assuntos: 3′-diaminobenzidine ; 5-bromo-4-chloro-3-indolyl-β-D-glucuronide ; ABC ; ABC transporter ; AHA ; Arabidopsis ; Arabidopsis - anatomy & histology ; Arabidopsis - cytology ; Arabidopsis - microbiology ; Arabidopsis - parasitology ; ARABIDOPSIS THALIANA ; ATP-binding cassette ; ATP-Binding Cassette Transporters - genetics ; ATP-Binding Cassette Transporters - metabolism ; Cell Death ; Cell Membrane - metabolism ; d.p.i ; DAB ; days post-inoculation ; DEFENCE MECHANISMS ; Exons - genetics ; GENE ; GENES ; Glucuronidase - metabolism ; GUS ; hypersensitive response ; HYPERSENSITIVITY ; MDR ; MECANISME DE DEFENSE ; MECANISMOS DE DEFENSA ; MRP ; multidrug resistance-associated protein ; multidrug-resistant protein ; Mutation - genetics ; NBD ; Non-host resistance ; nucleotide-binding domain ; Oomycetes ; pathogenesis related ; PDR ; Phytophthora - physiology ; Plant Diseases - microbiology ; Plant Diseases - parasitology ; Plant Leaves - cytology ; Plant Leaves - microbiology ; Plant Leaves - parasitology ; Plant Proteins - genetics ; plasma membrane H+-ATPase ; pleiotropic drug resistance ; Promoter Regions, Genetic - genetics ; Protein Transport ; PSEUDOMONAS SYRINGAE ; Pseudomonas syringae - physiology ; REACCIONES ALERGICAS ; REACTION ALLERGIQUE ; reverse transcription–PCR ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RT–PCR ; SAR ; Seedlings - cytology ; Seedlings - microbiology ; subunit a of the vacuolar H+-ATPase ; systemic aquired resistance ; TMD ; transmembrane domain ; vacuolar processing enzyme ; VHA-a ; Virulence ; VPE ; X-Gluc ; β-glucuronidase
É parte de: Plant and cell physiology, 2006-03, Vol.47 (3), p.309-318
Notas: 2007007786
F30
H20
local:pcj001
ark:/67375/HXZ-8W1W6WHG-Q
istex:7B9369D817E873278DCDB4B90A24BA8A89B40568
Corresponding author: E-mail, maeshima@agr.nagoya-u.ac.jp; Fax, +81-52-789-4096.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Plants contain a large number of ATP-binding cassette (ABC) transporters belonging to different subclasses. AtPDR8 is the only member of the pleiotropic drug resistance (PDR) ABC transporter subclass in Arabidopsis that is constitutively highly expressed. In transgenic Arabidopsis plants harboring the AtPDR8 promoter fused to β-glucuronidase (GUS), reporter expression was shown to be strong in the stomata and hydathode. In the stomata, transcripts of AtPDR8 were particularly frequent in the cells surrounding air spaces. Subcellular fractionation and immunochemical analysis showed that AtPDR8 was localized in the plasma membrane. When a knockout mutant of AtPDR8 (atpdr8) was infected with bacterial and oomycete pathogens, the plants exhibited chlorotic lesions and a hypersensitive response (HR)-like cell death. Cell death was detected in the atpdr8 mutants within 10 h of infection with the virulent bacterial pathogen, Pseudomonas syringae. As a result, the growth of P. syringae in the leaves of the atpdr8 mutant was reduced to 1% of that in the wild type. The defense response genes, PR-1, PR-2, PR-5, VPEγ, AtrbohD and AtrbohF were highly expressed when the mutant plants were grown under non-sterile conditions. The expression of the AtPDR8 gene was enhanced by infection of virulent and avirulent bacterial pathogens. Our results indicate that AtPDR8 is a key factor controlling the extent of cell death in the defense response and suggest that AtPDR8 transports some substance(s) which is closely related to the response of plants to pathogens.
Editor: Japan: Oxford University Press
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 10 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection

Kobae, Y.(Nagoya Univ. (Japan)) ; Sekino, T ; Yoshioka, H ; Nakagawa, T ; Martinoia, E ; Maeshima, M

Japan: Oxford University Press

Buscando em bases de dados remotas. Favor aguardar.