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Methylation of RAS association domain family protein 1A as a biomarker of lung cancer

Grote, Hans Juergen ; Schmiemann, Viola ; Geddert, Helene ; Bocking, Alfred ; Kappes, Rainer ; Gabbert, Helmut Erich ; Sarbia, Mario

Cancer, 2006-04, Vol.108 (2), p.129-134 [Peer Reviewed Journal]

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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Title:
Methylation of RAS association domain family protein 1A as a biomarker of lung cancer
Author: Grote, Hans Juergen ; Schmiemann, Viola ; Geddert, Helene ; Bocking, Alfred ; Kappes, Rainer ; Gabbert, Helmut Erich ; Sarbia, Mario
Subjects: Adenocarcinoma - chemistry ; Adenocarcinoma - diagnosis ; Adenocarcinoma - genetics ; Adenocarcinoma - pathology ; Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Biomarkers, Tumor - analysis ; Biomarkers, Tumor - genetics ; Biopsy, Needle ; Carcinoma, Non-Small-Cell Lung - chemistry ; Carcinoma, Non-Small-Cell Lung - diagnosis ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - pathology ; Carcinoma, Small Cell - chemistry ; Carcinoma, Small Cell - diagnosis ; Carcinoma, Small Cell - genetics ; Carcinoma, Small Cell - pathology ; Case-Control Studies ; Cell Line, Tumor ; Diagnosis, Differential ; DNA Methylation ; DNA, Neoplasm - analysis ; DNA, Neoplasm - genetics ; Female ; Gene Silencing ; Humans ; lung cancer ; Lung Neoplasms - chemistry ; Lung Neoplasms - diagnosis ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; Male ; Medical sciences ; Middle Aged ; molecular diagnostic techniques ; Molecular Diagnostic Techniques - methods ; Pneumology ; promoter methylation ; Promoter Regions, Genetic ; Retrospective Studies ; Reverse Transcriptase Polymerase Chain Reaction ; sensitivity ; Sensitivity and Specificity ; specificity ; Tumor Suppressor Proteins - analysis ; Tumor Suppressor Proteins - genetics ; Tumors ; Tumors of the respiratory system and mediastinum
Is Part Of: Cancer, 2006-04, Vol.108 (2), p.129-134
Notes: The first two authors contributed equally to this work.
Fax: (011) 49‐211‐8118402
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Description: BACKGROUND Promoter hypermethylation is an important mechanism for silencing tumor‐suppressor genes in cancer and a promising tool for development of molecular biomarkers. This study aimed to determine the prevalence of RAS association domain family protein 1A (RASSF1A) promoter hypermethylation in bronchial aspirates of patients with suspected lung cancer and to test whether this type of methylation assay could be used as a diagnostic adjunct to conventional cytology. METHODS Two hundred three bronchial aspirates from patients with suspected lung cancer were analyzed for RASSF1A hypermethylation by using a sensitive quantitative methylation‐specific polymerase chain reaction (QMSP). RESULTS RASSF1A hypermethylation was found in 88% (35 of 40), 28% (31 of 111), and 100% (6 of 6) of bronchial aspirates collected from patients diagnosed with small cell lung cancer, nonsmall cell lung cancer, and combined small cell lung cancer, respectively. No hypermethylation was detected in patients diagnosed with nonneoplastic lung disease (0 of 46). Depending on histologic subtype, up to 82% of cases presenting with a negative histology showed a positive methylation assay. CONCLUSIONS The QMSP analysis of RASSF1A hypermethylation enabled a highly specific distinction between patients diagnosed with lung cancer and those with nonneoplastic lung disease. These results suggested that a QMSP assay is a promising molecular tool for diagnosis of primary lung cancer. Cancer (Cancer Cytopathol) 2006. © 2006 American Cancer Society. Bronchial aspirates of patients with suspected lung cancer were investigated for the methylation status of the promoter region of RASSF1A by using a methylation‐specific real‐time PCR. RASSF1A hypermethylation appeared to be a useful biomarker for distinguishing patients with lung cancer from those with nonneoplastic lung disease.
Publisher: Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Language: English

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Methylation of RAS association domain family protein 1A as a biomarker of lung cancer

Grote, Hans Juergen ; Schmiemann, Viola ; Geddert, Helene ; Bocking, Alfred ; Kappes, Rainer ; Gabbert, Helmut Erich ; Sarbia, Mario

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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