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Effects of acetylcholine on inositol 1,4,5-triphosphate formation in cervix uteri myometrial cells of human

Nie, H ; Wei, M J ; Gao, M Q ; Ding, X P ; Li, Z

Zhōngguó yàolĭ xuébào, 1999-07, Vol.20 (7), p.659-662 [Periódico revisado por pares]

China

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Título:
Effects of acetylcholine on inositol 1,4,5-triphosphate formation in cervix uteri myometrial cells of human
Autor: Nie, H ; Wei, M J ; Gao, M Q ; Ding, X P ; Li, Z
Assuntos: Acetylcholine - pharmacology ; Adult ; Atropine - pharmacology ; Cell Separation ; Female ; Humans ; Inositol 1,4,5-Trisphosphate - biosynthesis ; Muscarinic Antagonists - pharmacology ; Myometrium - cytology ; Myometrium - metabolism ; Oxytocin - pharmacology ; Pregnancy - metabolism ; Vasodilator Agents - pharmacology
É parte de: Zhōngguó yàolĭ xuébào, 1999-07, Vol.20 (7), p.659-662
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Descrição: To detect inositol 1,4,5-triphosphate (IP3) formation of pregnant and nonpregnant human myometrial cells induced by acetylcholine (ACh). [3H] IP3 competitive protein binding assay. Basal levels of IP3 in pregnant and nonpregnant human myometrial cells were (82 +/- 9) and (96 +/- 10) nmol.g-1 (protein), respectively (n = 6). Incubated with ACh 50 mumol.L-1 for 5 min, IP3 reached the peak levels (109 +/- 11) and (122 +/- 15) nmol.g-1 (protein), respectively (n = 6), but difference of the increments of IP3 in pregnant and nonpregnant women was not significant. The increased IP3 induced by ACh was inhibited by atropine (Atr) 1 mumol.L-1. The basal IP3 level in pregnant cervix myometrial cells was higher than that in nonpregnant women. ACh increased the IP3 formation.
Editor: China
Idioma: Chinês

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Effects of acetylcholine on inositol 1,4,5-triphosphate formation in cervix uteri myometrial cells of human

Nie, H ; Wei, M J ; Gao, M Q ; Ding, X P ; Li, Z

China

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