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Improvement of Porous β-TCP Scaffolds with rhBMP-2 Chitosan Carrier Film for Bone Tissue Application

Abarrategi, Ander ; Moreno-Vicente, Carolina ; Ramos, Viviana ; Aranaz, Inmaculada ; Sanz Casado, José Vicente ; López-Lacomba, José Luís

Tissue engineering. Part A, 2008-08, Vol.14 (8), p.135-1319 [Periódico revisado por pares]

United States: Mary Ann Liebert, Inc

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Improvement of Porous β-TCP Scaffolds with rhBMP-2 Chitosan Carrier Film for Bone Tissue Application
Autor: Abarrategi, Ander ; Moreno-Vicente, Carolina ; Ramos, Viviana ; Aranaz, Inmaculada ; Sanz Casado, José Vicente ; López-Lacomba, José Luís
Assuntos: Animals ; Bone and Bones - drug effects ; Bone cells ; Bone Morphogenetic Protein 2 ; Bone morphogenetic proteins ; Bone Morphogenetic Proteins - pharmacology ; Calcium Phosphates - pharmacology ; Cell Differentiation - drug effects ; Cell Line ; Cell Proliferation - drug effects ; Ceramic materials ; Ceramics ; Ceramics - pharmacology ; Chitosan - pharmacology ; Genetic aspects ; Humans ; Materials Testing ; Methods ; Mice ; Microscopy, Electron, Scanning ; Myoblasts - cytology ; Myoblasts - drug effects ; Myoblasts - ultrastructure ; Physiological aspects ; Porosity ; Rabbits ; Recombinant Proteins - pharmacology ; Skull - abnormalities ; Spectroscopy, Fourier Transform Infrared ; Tissue Engineering ; Tissue Scaffolds ; Tomography, X-Ray Computed ; Transforming Growth Factor beta - pharmacology ; X-Ray Diffraction
É parte de: Tissue engineering. Part A, 2008-08, Vol.14 (8), p.135-1319
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Descrição: Ceramic materials are osteoconductive matrices extensively used in bone tissue engineering approaches. The performance of these types of biomaterials can be greatly enhanced by the incorporation of bioactive agents and materials. It is previously reported that chitosan is a biocompatible, biodegradable material that enhances bone formation. In the other hand, bone morphogenetic protein-2 (BMP-2) is a well-known osteoinductive factor. In this work we coated porous β-tricalcium phosphate (β-TCP) scaffolds with recombinant human BMP-2 (rhBMP-2) carrier chitosan films and studied how they could modify the ceramic physicochemical properties, cellular response, and in vivo bone generation. Initial β-TCP disks with an average diameter of 5.78 mm, 2.9 mm thickness, and 53% porosity were coated with a chitosan film. These coating properties were studied by X-ray diffraction, Fourier transform-infrared analysis, transmission electron microscopy, scanning electron microscopy, and energy dispersive X-ray analysis (EDX). Treatment modified the scaffold porous distribution and increased the average hardness. The biocompatibility did not seem to be altered. In addition, adhered C2C12 cells expressed alkaline phosphatase activity, related to cell differentiation toward osteogenic lineage, due to the incorporation of rhBMP-2. On the other hand, in vivo observations showed new bone formation 3 weeks after surgery, a much shorter time than control β-TCP ceramics. These results suggest that developed coating improved porous β-TCP scaffold for bone tissue applications and added osteoinductive properties.
Editor: United States: Mary Ann Liebert, Inc
Idioma: Inglês

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Improvement of Porous β-TCP Scaffolds with rhBMP-2 Chitosan Carrier Film for Bone Tissue Application

Abarrategi, Ander ; Moreno-Vicente, Carolina ; Ramos, Viviana ; Aranaz, Inmaculada ; Sanz Casado, José Vicente ; López-Lacomba, José Luís

United States: Mary Ann Liebert, Inc

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