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Cytotoxicity of Octal-Bromide Zinc Phthalocyanine After Photodynamic Therapy with Different Light Sources

Machado, Aline Helena Araujo ; Soares, Cristina Pacheco ; Junior, Milton Beltrame ; Pacheco, Marcos Tadeu Tavares ; Da Silva, Newton Soares

Photomedicine and laser surgery, 2008-10, Vol.26 (5), p.455-459 [Periódico revisado por pares]

United States: Mary Ann Liebert, Inc

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Título:
Cytotoxicity of Octal-Bromide Zinc Phthalocyanine After Photodynamic Therapy with Different Light Sources
Autor: Machado, Aline Helena Araujo ; Soares, Cristina Pacheco ; Junior, Milton Beltrame ; Pacheco, Marcos Tadeu Tavares ; Da Silva, Newton Soares
Assuntos: Cancer ; Causes of ; Cell Culture Techniques ; Cell Line, Tumor - drug effects ; Cell Line, Tumor - radiation effects ; Cell Survival - drug effects ; Cell Survival - radiation effects ; Cell-mediated cytotoxicity ; Complications and side effects ; Control ; Epithelial Cells - drug effects ; Epithelial Cells - pathology ; Epithelial Cells - radiation effects ; Health aspects ; Humans ; Optical Devices ; Organometallic Compounds - pharmacology ; Photochemotherapy ; Photochemotherapy - instrumentation ; Photosensitizing Agents - pharmacology ; Phthalocyanins ; Semiconductor lasers
É parte de: Photomedicine and laser surgery, 2008-10, Vol.26 (5), p.455-459
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Objective: The objective of this study was to investigate the cytotoxicity of octal-bromide zinc phthalocyanine (ZnPcBr 8 ) at different concentrations (0.25, 0.5, and 1 μ M) after irradiating HEp-2 cell cultures with two different light sources: a diode semiconductor laser (660 nm, 30 mW) or an LED (640 nm, 70 mW). In order to obtain comparative results, the irradiation parameters of both light sources were adjusted so that the amount of energy density delivered would be the same (4.5 J/cm 2 ). Background Data: Numerous photosensitizers and light sources used in the treatment of human disease have been studied. Based on these studies, a comparative evaluation of two light sources used in photodynamic therapy (PDT) with ZnPcBr 8 was proposed. Materials and Methods: HEp-2 cells were incubated with ZnPcBr 8 at different concentrations (0.25, 0.5, or 1 μ M) for 1 h, irradiated with the diode semiconductor laser (660 nm at 30 mW for 300 sec; 4.5 J/cm 2 ) or the LED laser (640 nm at 70 mW for 128 sec; 4.5 J/cm 2 ), and then incubated in MEM medium for 1 or 24 h. The cells were analyzed using the MTT and trypan blue dye exclusion tests. Results: The results demonstrated that the concentration of 1 μ M of ZnPcBr 8 was the most effective after PDT administered by both light sources. According to the MTT results, HEp-2-cell viability decreased by 97.96% 1 h after, and by 99.87% 24 h after irradiation with the diode semiconductor laser, and decreased by 94.03% 1 h after, and by 99.21% 24 h after irradiation with the LED. The results obtained using the trypan blue dye exclusion test confirmed the photodynamic efficacy of ZnPcBr 8 employed with both light sources. With regard to HEp-2-cell viability, the following results were observed: a decrease of 98.73% 1 h after, and of 99.49% 24 h after irradiation with the diode semiconductor laser; and a decrease of 98.76% 1 h after, and of 99.23% 24 h after irradiation with the LED. Conclusions: According to our results with the irradiation parameters studied here, both the LED and diode semiconductor laser can be used for PDT in vitro , since both light sources had excellent photodynamic efficacy.
Editor: United States: Mary Ann Liebert, Inc
Idioma: Inglês

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Cytotoxicity of Octal-Bromide Zinc Phthalocyanine After Photodynamic Therapy with Different Light Sources

Machado, Aline Helena Araujo ; Soares, Cristina Pacheco ; Junior, Milton Beltrame ; Pacheco, Marcos Tadeu Tavares ; Da Silva, Newton Soares

United States: Mary Ann Liebert, Inc

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