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Glucose-6-phosphate dehydrogenase plays a pivotal role in nitric oxide-involved defense against oxidative stress under salt stress in red kidney bean roots [Phaseolus vulgaris]

Liu, Y.(Lanzhou Univ. (China)) ; Wu, R ; Wan, Q ; Xie, G ; Bi, Y

Plant and cell physiology, 2007-03, Vol.48 (3), p.511-522 [Periódico revisado por pares]

Japan: Oxford University Press

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Glucose-6-phosphate dehydrogenase plays a pivotal role in nitric oxide-involved defense against oxidative stress under salt stress in red kidney bean roots [Phaseolus vulgaris]
Autor: Liu, Y.(Lanzhou Univ. (China)) ; Wu, R ; Wan, Q ; Xie, G ; Bi, Y
Assuntos: ANTIOXIDANTES ; ANTIOXIDANTS ; ANTIOXYDANT ; Cell Membrane Permeability ; CHLORURE DE SODIUM ; CLORURO SODICO ; ESTRES ; EXPRESION GENICA ; EXPRESSION DES GENES ; GENE EXPRESSION ; GLUCOSA 6 FOSFATO DESHIDROGENASA ; GLUCOSE 6 PHOSPHATE DEHYDROGENASE ; GLUCOSE 6 PHOSPHATE DESHYDROGENASE ; Glucosephosphate Dehydrogenase - metabolism ; Models, Biological ; NITRATE REDUCTASE ; Nitrate Reductase - metabolism ; NITRATO REDUCTASA ; NITRIC OXIDE ; Nitric Oxide - metabolism ; Oxidation-Reduction ; Oxidative Stress ; OXIDO NITRICO ; OXYDE NITRIQUE ; Phaseolus - drug effects ; Phaseolus - metabolism ; PHASEOLUS VULGARIS ; Plant Roots - drug effects ; Plant Roots - metabolism ; SODIUM CHLORIDE ; Sodium Chloride - pharmacology ; STRESS ; Thiobarbituric Acid Reactive Substances - metabolism
É parte de: Plant and cell physiology, 2007-03, Vol.48 (3), p.511-522
Notas: 2007008922
H50
F40
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
Descrição: The pivotal role of glucose-6-phosphate dehydrogenase (G-6-PDH)-mediated nitric oxide (NO) production in the tolerance to oxidative stress induced by 100 mM NaCl in red kidney bean (Phaseolus vulgaris) roots was investigated. The results show that the G-6-PDH activity was enhanced rapidly in the presence of NaCl and reached a maximum at 100 mM. Western blot analysis indicated that the increase of G-6-PDH activity in the red kidney bean roots under 100 mM NaCl was mainly due to the increased content of the G-6-PDH protein. NO production and nitrate reductase (NR) activity were also induced by 100 mM NaCl. The NO production was reduced by NaN3 (an NR inhibitor), but not affected by Nomega-nitro-L-arginine (L-NNA) (an NOS inhibitor). Application of 2.5 mM Na3PO4, an inhibitor of G-6-PDH, blocked the increase of G-6-PDH and NR activity, as well as NO production in red kidney bean roots under 100 mM NaCl. The activities of antioxidant enzymes in red kidney bean roots increased in the presence of 100 mM NaCl or sodium nitroprusside (SNP), an NO donor. The increased activities of all antioxidant enzymes tested at 100 mM NaCl were completely inhibited by 2.5 mM Na3PO4. Based on these results, we conclude that G-6-PDH plays a pivotal role in NR-dependent NO production, and in establishing tolerance of red kidney bean roots to salt stress.
Editor: Japan: Oxford University Press
Idioma: Inglês

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Glucose-6-phosphate dehydrogenase plays a pivotal role in nitric oxide-involved defense against oxidative stress under salt stress in red kidney bean roots [Phaseolus vulgaris]

Liu, Y.(Lanzhou Univ. (China)) ; Wu, R ; Wan, Q ; Xie, G ; Bi, Y

Japan: Oxford University Press

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