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B-50/GAP-43 Potentiates Cytoskeletal Reorganization in Raft Domains

Aarts, Lambertus H.J. ; Verkade, Paul ; van Dalen, Jacqueline J.W. ; van Rozen, Andrea J. ; Gispen, Willem Hendrik ; Schrama, Loes H. ; Schotman, Peter

Molecular and cellular neuroscience, 1999-08, Vol.14 (2), p.85-97 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
B-50/GAP-43 Potentiates Cytoskeletal Reorganization in Raft Domains
Autor: Aarts, Lambertus H.J. ; Verkade, Paul ; van Dalen, Jacqueline J.W. ; van Rozen, Andrea J. ; Gispen, Willem Hendrik ; Schrama, Loes H. ; Schotman, Peter
Assuntos: Animals ; Cell Line ; Cell Membrane - ultrastructure ; Cells, Cultured ; Cytoskeleton - physiology ; Cytoskeleton - ultrastructure ; DNA, Complementary ; Embryo, Mammalian ; Fibroblasts ; GAP-43 Protein - genetics ; GAP-43 Protein - metabolism ; Glycosylphosphatidylinositols - metabolism ; Hippocampus - cytology ; Hippocampus - metabolism ; Immunohistochemistry ; Neurons - metabolism ; Neurons - ultrastructure ; Organelles - metabolism ; Organelles - ultrastructure ; Rats ; Recombinant Fusion Proteins - metabolism ; Thy-1 Antigens - analysis ; Transfection
É parte de: Molecular and cellular neuroscience, 1999-08, Vol.14 (2), p.85-97
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: B-50 (GAP-43) is a neural, membrane-associated protein that has been implicated in neurite outgrowth and guidance. Following stable transfection of Rat1 fibroblasts with B-50 cDNA we observed a dispersed distribution of B-50 immunoreactivity in flattened resting cells. In contrast, motile cells exhibited high concentrations of B-50 at the leading edge of ruffling membranes, coinciding with actin polymerization. Time-lapse studies on Rat1 fibroblasts transiently transfected with B-50/EGFP revealed that large vesicles originated from the ruffling membranes. These large vesicles (pinocytes) were found positive for Thy-1, a GPI-anchored protein, but negative for rab-5, an early endosome marker. In primary hippocampal neurons B-50 also colocalized completely with the raft marker Thy-1. Antibody-mediated cross-linking of Thy-1 in hippocampal neurons resulted in a redistribution of the intracellular protein B-50 to Thy-1-immunopositive membrane patches, whereas syntaxin was mainly excluded from the patches, showing that B-50 is associated with rafts.
Editor: United States: Elsevier Inc
Idioma: Inglês

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B-50/GAP-43 Potentiates Cytoskeletal Reorganization in Raft Domains

Aarts, Lambertus H.J. ; Verkade, Paul ; van Dalen, Jacqueline J.W. ; van Rozen, Andrea J. ; Gispen, Willem Hendrik ; Schrama, Loes H. ; Schotman, Peter

United States: Elsevier Inc

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