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Strain differences in urocortin expression in the Edinger–Westphal nucleus and its relation to alcohol-induced hypothermia

Bachtell, R.K ; Tsivkovskaia, N.O ; Ryabinin, A.E

Neuroscience, 2002-01, Vol.113 (2), p.421-434 [Periódico revisado por pares]

Oxford: Elsevier Ltd

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Título:
Strain differences in urocortin expression in the Edinger–Westphal nucleus and its relation to alcohol-induced hypothermia
Autor: Bachtell, R.K ; Tsivkovskaia, N.O ; Ryabinin, A.E
Assuntos: Alcoholism and acute alcohol poisoning ; Animals ; Behavior, Animal - physiology ; Biological and medical sciences ; Brain - drug effects ; Brain - metabolism ; c-Fos ; C57BL/6 ; Corticotropin-Releasing Hormone - metabolism ; DBA/2 ; Dose-Response Relationship, Drug ; Drug Administration Schedule ; Ethanol - administration & dosage ; Ethanol - metabolism ; Ethanol - pharmacology ; Female ; HOT/COLD ; Hypothermia - chemically induced ; Hypothermia - genetics ; Hypothermia - metabolism ; Hypothermia - pathology ; Immunohistochemistry ; inbred strains ; Male ; Medical sciences ; Mice ; Mice, Inbred C57BL - genetics ; Mice, Inbred DBA - genetics ; Neurons - metabolism ; Proto-Oncogene Proteins c-fos - metabolism ; selected lines ; Sex Characteristics ; Species Specificity ; Tissue Distribution ; Toxicology ; Urocortins
É parte de: Neuroscience, 2002-01, Vol.113 (2), p.421-434
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: The Edinger–Westphal nucleus is the primary source of urocortin in rodent brain. Mapping of inducible transcription factors has shown that the Edinger–Westphal nucleus is preferentially sensitive to ethanol self-administration. In the present study we have immunohistochemically compared expression of urocortin and c-Fos in naive and ethanol-treated C57BL/6J and DBA/2J mouse inbred strains. We found that C57BL/6J mice possess significantly higher numbers of urocortin-expressing cells in the Edinger–Westphal compared to DBA/2J mice. Subsequent histological analysis confirmed a lower number of large neurons in the DBA/2J Edinger–Westphal nucleus. Surprisingly, despite the differences in structure, no strain differences were observed in the number of c-Fos-containing cells after acute (0.6–4.8 g/kg, i.p.) and repeated (2.4 g/kg, 14 days, one injection/day) administration of ethanol. Double-label immunohistochemistry showed that ethanol-induced c-Fos expression is present in different sets of Edinger–Westphal cells between the strains. Specifically, expression of c-Fos in C57BL/6J mice is preferentially induced in urocortin cells, while c-Fos in DBA/2J mice occurs in a mixed population of cells. Behavioral analysis of the B6D2 F2 intercross, a heterogeneous mouse strain, showed that the number of urocortin cells is positively correlated with basal temperatures and ethanol-induced hypothermia. Involvement of the Edinger–Westphal in alcohol-induced hypothermia is further confirmed by analysis of urocortin cells in the HOT/COLD selected lines. These results provide evidence that C57BL/6J and DBA/2J mice have structural differences in the Edinger–Westphal that can result in activation of different populations of neurons upon alcohol intoxication contributing to differential thermoregulation between these inbred strains.
Editor: Oxford: Elsevier Ltd
Idioma: Inglês

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Strain differences in urocortin expression in the Edinger–Westphal nucleus and its relation to alcohol-induced hypothermia

Bachtell, R.K ; Tsivkovskaia, N.O ; Ryabinin, A.E

Oxford: Elsevier Ltd

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