Idioma:

Copper-free click chemistry in living animals

Chang, Pamela V ; Prescher, Jennifer A ; Sletten, Ellen M ; Baskin, Jeremy M ; Miller, Isaac A ; Agard, Nicholas J ; Lo, Anderson ; Bertozzi, Carolyn R

Proceedings of the National Academy of Sciences - PNAS, 2010-02, Vol.107 (5), p.1821-1826 [Periódico revisado por pares]

United States: National Academy of Sciences

Texto completo disponível

Citações Citado por

Enviar para

Título:
Copper-free click chemistry in living animals
Autor: Chang, Pamela V ; Prescher, Jennifer A ; Sletten, Ellen M ; Baskin, Jeremy M ; Miller, Isaac A ; Agard, Nicholas J ; Lo, Anderson ; Bertozzi, Carolyn R
Assuntos: Animals ; Antibodies ; Azides ; Azides - chemistry ; Azides - metabolism ; Cell Membrane - metabolism ; Chemical reactions ; Click chemistry ; Copper ; Copper - chemistry ; Copper - metabolism ; Cyclization ; Cycloaddition ; Cyclooctanes - chemistry ; Cyclooctanes - metabolism ; Glycoconjugates - chemistry ; Glycoconjugates - metabolism ; Hexosamines - chemistry ; Hexosamines - metabolism ; Humans ; In Vitro Techniques ; Indicators and Reagents ; Jurkat Cells ; Ligation ; Liver ; Mice ; Models, Biological ; Molecular biology ; Molecular Probes - chemistry ; Molecules ; Physical Sciences ; Protein Binding ; Reactivity ; Reagents ; Rodents ; Serum Albumin - metabolism ; Spleen - cytology ; Spleen - metabolism ; Splenocytes ; Vehicles
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2010-02, Vol.107 (5), p.1821-1826
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Edited by M.G. Finn, The Scripps Research Institute, La Jolla, CA, and accepted by the Editorial Board December 9, 2009 (received for review September 25, 2009)
1P.V.C. and J.A.P. contributed equally to this work.
Author contributions: P.V.C., J.A.P., J.M.B., and C.R.B. designed research; P.V.C., J.A.P., E.M.S., J.M.B., and I.A.M. performed research; P.V.C., J.A.P., E.M.S., J.M.B., I.A.M., and C.R.B. analyzed data; N.J.A. and A.L. contributed new reagents/analytic tools; and P.V.C. and C.R.B. wrote the paper.
Descrição: Chemical reactions that enable selective biomolecule labeling in living organisms offer a means to probe biological processes in vivo. Very few reactions possess the requisite bioorthogonality, and, among these, only the Staudinger ligation between azides and triarylphosphines has been employed for direct covalent modification of biomolecules with probes in the mouse, an important model organism for studies of human disease. Here we explore an alternative bioorthogonal reaction, the 1,3-dipolar cycloaddition of azides and cyclooctynes, also known as "Cu-free click chemistry," for labeling biomolecules in live mice. Mice were administered peracetylated N-azidoacetylmannosamine (Ac₄ManNAz) to metabolically label cell-surface sialic acids with azides. After subsequent injection with cyclooctyne reagents, glycoconjugate labeling was observed on isolated splenocytes and in a variety of tissues including the intestines, heart, and liver, with no apparent toxicity. The cyclooctynes tested displayed various labeling efficiencies that likely reflect the combined influence of intrinsic reactivity and bioavailability. These studies establish Cu-free click chemistry as a bioorthogonal reaction that can be executed in the physiologically relevant context of a mouse.
Editor: United States: National Academy of Sciences
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 9 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Copper-free click chemistry in living animals

Chang, Pamela V ; Prescher, Jennifer A ; Sletten, Ellen M ; Baskin, Jeremy M ; Miller, Isaac A ; Agard, Nicholas J ; Lo, Anderson ; Bertozzi, Carolyn R

United States: National Academy of Sciences

Buscando em bases de dados remotas. Favor aguardar.