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Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii

SU, C. ; SHWAB, E. K. ; ZHOU, P. ; ZHU, X. Q. ; DUBEY, J. P.

Parasitology, 2010-01, Vol.137 (1), p.1-11 [Periódico revisado por pares]

Cambridge, UK: Cambridge University Press

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Título:
Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii
Autor: SU, C. ; SHWAB, E. K. ; ZHOU, P. ; ZHU, X. Q. ; DUBEY, J. P.
Assuntos: Animals ; Biological and medical sciences ; disease diagnosis ; DNA, Protozoan - analysis ; DNA, Protozoan - genetics ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; genetic characterization ; Genetic Techniques ; genetic techniques and protocols ; Genotype ; Humans ; Invertebrates ; literature reviews ; molecular detection and identification ; molecular genetics ; multilocus sequence typing ; multiplex polymerase chain reaction ; nested polymerase chain reaction ; pathogen identification ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Protozoan Proteins - genetics ; restriction fragment length polymorphism ; Sequence Analysis, DNA - methods ; Toxoplasma - classification ; Toxoplasma - genetics ; Toxoplasma - isolation & purification ; Toxoplasma gondii ; toxoplasmosis ; Toxoplasmosis - diagnosis ; Toxoplasmosis - epidemiology ; Toxoplasmosis - parasitology
É parte de: Parasitology, 2010-01, Vol.137 (1), p.1-11
Notas: http://dx.doi.org/10.1017/S0031182009991065
http://hdl.handle.net/10113/47133
ArticleID:99106
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PII:S0031182009991065
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ObjectType-Feature-1
content type line 23
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ObjectType-Review-1
Descrição: The development of simple, sensitive and rapid methods for the detection and identification of Toxoplasma gondii is important for the diagnosis and epidemiological studies of the zoonotic disease toxoplasmosis. In the past 2 decades, molecular methods based on a variety of genetic markers have been developed, each with its advantages and limitations. The application of these methods has generated invaluable information to enhance our understanding of the epidemiology, population genetics and phylogeny of T. gondii. However, since most studies focused solely on the detection but not genetic characterization of T. gondii, the information obtained was limited. In this review, we discuss some widely used molecular methods and propose an integrated approach for the detection and identification of T. gondii, in order to generate maximum information for epidemiological, population and phylogenetic studies of this key pathogen.
Editor: Cambridge, UK: Cambridge University Press
Idioma: Inglês

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Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii

SU, C. ; SHWAB, E. K. ; ZHOU, P. ; ZHU, X. Q. ; DUBEY, J. P.

Cambridge, UK: Cambridge University Press

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