Language:

A simple method to introduce marker-free genetic modifications into the chromosome of naturally nontransformable Bacillus amyloliquefaciens strains

Zakataeva, Natalia P. ; Nikitina, Oksana V. ; Gronskiy, Sergey V. ; Romanenkov, Dmitriy V. ; Livshits, Vitaliy A.

Applied microbiology and biotechnology, 2010, Vol.85 (4), p.1201-1209 [Peer Reviewed Journal]

Berlin/Heidelberg: Springer-Verlag

Full text available

Citations Cited by

Actions
1. Add to e-Shelf
2. Remove from e-Shelf
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
A simple method to introduce marker-free genetic modifications into the chromosome of naturally nontransformable Bacillus amyloliquefaciens strains
Author: Zakataeva, Natalia P. ; Nikitina, Oksana V. ; Gronskiy, Sergey V. ; Romanenkov, Dmitriy V. ; Livshits, Vitaliy A.
Subjects: Antibiotics ; Bacillus ; Bacillus - genetics ; Bacillus - metabolism ; Bacillus amyloliquefaciens ; Bacteria ; Bacterial Proteins - genetics ; Biomedical and Life Sciences ; Biotechnology ; Chromosomes ; Chromosomes, Bacterial ; DNA polymerase ; DNA, Bacterial - genetics ; E coli ; Enzymes ; Escherichia coli - genetics ; Gene Deletion ; Genes, Bacterial ; Genetic engineering ; Genetic Markers ; Genetic Vectors ; Genetics ; Glucose 1-Dehydrogenase - genetics ; Life Sciences ; Medical research ; Membrane Proteins - genetics ; Metabolism ; Metabolites ; Methods ; Microbial Genetics and Genomics ; Microbiology ; Mutagenesis ; Mutagenesis, Insertional ; Mutagenesis, Site-Directed ; Mutation ; Physiology ; Plasmids ; Point Mutation ; Polymerase chain reaction ; Promoter Regions, Genetic ; Regulatory Sequences, Nucleic Acid ; Studies ; Transformation, Bacterial
Is Part Of: Applied microbiology and biotechnology, 2010, Vol.85 (4), p.1201-1209
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: A simple method to introduce marker-free deletions, insertions, and point mutations into the chromosomes of naturally nontransformable Bacillus amyloliquefaciens strains has been developed. The method is efficient and fast, and it allows for the generation of genetic modifications without the use of a counter-selectable marker or a special prerequisite strain. This method uses the combination of the following: the effective introduction of a delivery plasmid into cells for gene replacement; a two-step replacement recombination procedure, which occurs at a very high frequency due to the use of a thermosensitive rolling-circle replication plasmid; and colony polymerase chain reaction (PCR) analysis for screening. Using PCR primers with mismatches at the 3′ end enables the selection of strains that contain a single nucleotide substitution in the target gene. This approach can be used as a routine method for the investigation of complex physiological pathways and for the metabolic engineering of food-grade industrial B. amyloliquefaciens and other Bacillus strains.
Publisher: Berlin/Heidelberg: Springer-Verlag
Language: English;Russian

Links

Back to results list

Previous Result 3 Next Go to Next page

Implemented by: Social Network Logos:

A simple method to introduce marker-free genetic modifications into the chromosome of naturally nontransformable Bacillus amyloliquefaciens strains

Zakataeva, Natalia P. ; Nikitina, Oksana V. ; Gronskiy, Sergey V. ; Romanenkov, Dmitriy V. ; Livshits, Vitaliy A.

Berlin/Heidelberg: Springer-Verlag

Searching Remote Databases, Please Wait