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USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis

Groettrup, Marcus ; Aichem, Annette ; Pelzer, Christiane ; Lukasiak, Sebastian ; Kalveram, Birte ; Sheppard, Paul W ; Rani, Neha ; Schmidtke, Gunter

Nature communications, 2010-05, Vol.1 (2), p.1-10, Article 13 [Revista revisada por pares]

England: Nature Publishing Group

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USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis
Autor: Groettrup, Marcus ; Aichem, Annette ; Pelzer, Christiane ; Lukasiak, Sebastian ; Kalveram, Birte ; Sheppard, Paul W ; Rani, Neha ; Schmidtke, Gunter
Materias: HEK293 Cells ; Humans ; Protein Binding - genetics ; Protein Binding - physiology ; RNA, Small Interfering ; Two-Hybrid System Techniques ; Ubiquitin - genetics ; Ubiquitin - metabolism ; Ubiquitins - genetics ; Ubiquitins - metabolism
Es parte de: Nature communications, 2010-05, Vol.1 (2), p.1-10, Article 13
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descripción: The ubiquitin-like modifier FAT10 targets proteins for degradation by the proteasome and is activated by the E1 enzyme UBA6. In this study, we identify the UBA6-specific E2 enzyme (USE1) as an interaction partner of FAT10. Activated FAT10 can be transferred from UBA6 onto USE1 in vitro, and endogenous USE1 and FAT10 can be coimmunoprecipitated from intact cells. Small interfering RNA-mediated downregulation of USE1 mRNA resulted in a strong reduction of FAT10 conjugate formation under endogenous conditions, suggesting that USE1 is a major E2 enzyme in the FAT10 conjugation cascade. Interestingly, USE1 is not only the first E2 enzyme but also the first known substrate of FAT10 conjugation, as it was efficiently auto-FAT10ylated in cis but not in trans.
Editor: England: Nature Publishing Group
Idioma: Inglés

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USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis

Groettrup, Marcus ; Aichem, Annette ; Pelzer, Christiane ; Lukasiak, Sebastian ; Kalveram, Birte ; Sheppard, Paul W ; Rani, Neha ; Schmidtke, Gunter

England: Nature Publishing Group

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