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Application of genomic DNA subtyping by pulsed field gel electrophoresis and restriction enzyme analysis of plasmid DNA to characterize methicillin-resistant Staphylococcus aureus from two nosocomial outbreaks

Branchini, Maria Luiza M. ; Morthland, Virginia H. ; Tresoldi, Antonia T. ; Von Nowakonsky, Angela ; Dias, Maria Beatriz Souza ; Pfaller, Michael A.

Diagnostic microbiology and infectious disease, 1993-11, Vol.17 (4), p.275-281 [Periódico revisado por pares]

New York, NY: Elsevier Inc

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Título:
Application of genomic DNA subtyping by pulsed field gel electrophoresis and restriction enzyme analysis of plasmid DNA to characterize methicillin-resistant Staphylococcus aureus from two nosocomial outbreaks
Autor: Branchini, Maria Luiza M. ; Morthland, Virginia H. ; Tresoldi, Antonia T. ; Von Nowakonsky, Angela ; Dias, Maria Beatriz Souza ; Pfaller, Michael A.
Assuntos: Bacterial Typing Techniques ; Bacteriology ; Biological and medical sciences ; Brazil - epidemiology ; Cross Infection - epidemiology ; Cross Infection - microbiology ; Disease Outbreaks ; DNA Restriction Enzymes - analysis ; DNA, Bacterial - analysis ; Electrophoresis, Gel, Pulsed-Field ; Fundamental and applied biological sciences. Psychology ; Genetics ; Humans ; Methicillin Resistance ; Microbiology ; Plasmids - genetics ; Species Specificity ; Staphylococcus aureus - classification ; Staphylococcus aureus - genetics
É parte de: Diagnostic microbiology and infectious disease, 1993-11, Vol.17 (4), p.275-281
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Pulsed-field gel electrophoresis (PFGE) and restriction enzyme analysis of plasmid DNA (REAP) were applied to study the epidemiologic relationship among methicillin-resistant Staphylococcus aureus (MRSA) isolates from outbreaks in two hospitals in São Paulo, Brazil: 82 MRSA isolates, 73 from a university hospital and nine from a general adult intensive care unit of a private hospital, were collected from 62 patients: 95% of the MRSAs were also resistant to gentamicin and ciprofloxacin. REAP subtyping of both collections identified six different subtypes: 55 (72.6%) MRSAs from the university hospital and nine isolates from the private hospital shared the same epidemic REAP subtype. Discrimination by restriction of genomic DNA with Sma I followed by PFGE enabled the identification of 14 DNA subtypes. Based on the combined REAP-genomic DNA subtype, the predominant subtype in the university hospital was A/A (44 isolates) whereas the epidemic subtype in the private hospital was A/M (seven isolates). The application of two typing methods showed better discrimination among MRSAs than did either method alone.
Editor: New York, NY: Elsevier Inc
Idioma: Inglês

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Application of genomic DNA subtyping by pulsed field gel electrophoresis and restriction enzyme analysis of plasmid DNA to characterize methicillin-resistant Staphylococcus aureus from two nosocomial outbreaks

Branchini, Maria Luiza M. ; Morthland, Virginia H. ; Tresoldi, Antonia T. ; Von Nowakonsky, Angela ; Dias, Maria Beatriz Souza ; Pfaller, Michael A.

New York, NY: Elsevier Inc

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