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Two alleles of the gene encoding surface antigen P22 in 25 strains of Toxoplasma gondii

Parmley, S.F ; Gross, U ; Sucharczuk, A ; Windeck, T ; Sgarlato, G.D ; Remington, J.S

The Journal of parasitology, 1994-04, Vol.80 (2), p.293-301 [Periódico revisado por pares]

United States: American Society of Parasitologists

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Título:
Two alleles of the gene encoding surface antigen P22 in 25 strains of Toxoplasma gondii
Autor: Parmley, S.F ; Gross, U ; Sucharczuk, A ; Windeck, T ; Sgarlato, G.D ; Remington, J.S
Assuntos: Alleles ; Animals ; Antibodies ; Antibodies, Monoclonal - immunology ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; ANTIGENE ; ANTIGENOS ; ANTIGENS ; Antigens, Protozoan - genetics ; Antigens, Protozoan - immunology ; Antigens, Surface - genetics ; Antigens, Surface - immunology ; Base Sequence ; BIOLOGICAL DIFFERENCES ; DIFERENCIAS BIOLOGICAS ; DIFFERENCE BIOLOGIQUE ; DNA ; DNA Primers - chemistry ; DNA, Protozoan - chemistry ; GENE ; Gene Expression Regulation ; GENES ; Genes, Protozoan ; Genetic loci ; Immunoblotting ; Mice ; Molecular Sequence Data ; Molecular-Cell Biology ; MONOCLONAL ANTIBODIES ; NUCLEOTIDE SEQUENCE ; Parasites ; Parasitology ; PATHOGENICITY ; PODER PATOGENO ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; POUVOIR PATHOGENE ; Protozoan Proteins ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; SECUENCIA NUCLEICA ; SEQUENCE NUCLEIQUE ; STRAIN DIFFERENCES ; Surface antigens ; Toxoplasma - genetics ; Toxoplasma - immunology ; Toxoplasma - pathogenicity ; TOXOPLASMA GONDII ; VIRULENCE ; Virulence - genetics ; Virulence - immunology
É parte de: The Journal of parasitology, 1994-04, Vol.80 (2), p.293-301
Notas: L72
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Descrição: A group of 25 strains of Toxoplasma gondii (4 mouse-virulent strains and 21 mouse-avirulent strains) were tested by immunoblot with 4 monoclonal antibodies (MAb) against surface antigen P22. Parasite lysates from only 12 strains were recognized by all 4 MAbs, while lysates from the remaining 13 strains (all avirulent) were not recognized by any of the 4 MAbs. Strains not recognized by the 4 MAbs were found to express an altered form of the P22 antigen. Sequencing of the P22 genes from 10 strains revealed only 2 alleles. One allele is identical to the gene from the virulent RH strain. The second allele carries 5 single nucleotide substitutions and an insertion of a GGT triplet when compared to the allele from the RH strain. Four of the 5 nucleotide changes result in amino acid substitutions and the triplet insertion results in an extra glycine residue. Four of the single base changes also result in restriction fragment length polymorphisms (RFLP). RFLP analysis of the P22 gene revealed only 2 patterns among the 25 strains. The allele of the P22 gene correlated with reactivity of the 4 MAbs to lysates of each strain. However, the allele of the P22 gene did not correlate with the virulence of each strain for mice.
Editor: United States: American Society of Parasitologists
Idioma: Inglês

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Two alleles of the gene encoding surface antigen P22 in 25 strains of Toxoplasma gondii

Parmley, S.F ; Gross, U ; Sucharczuk, A ; Windeck, T ; Sgarlato, G.D ; Remington, J.S

United States: American Society of Parasitologists

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