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Comparison of EIA, Culture, and Polymerase Chain Reaction for Chlamydia trachomatis in a Sexually Transmitted Disease Clinic

Sweet, Richard L. ; Wiesenfeld, Harold C. ; Uhrin, Michael ; Dixon, Bruce

The Journal of infectious diseases, 1994-08, Vol.170 (2), p.500-501 [Periódico revisado por pares]

United States: The University of Chicago Press

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  • Título:
    Comparison of EIA, Culture, and Polymerase Chain Reaction for Chlamydia trachomatis in a Sexually Transmitted Disease Clinic
  • Autor: Sweet, Richard L. ; Wiesenfeld, Harold C. ; Uhrin, Michael ; Dixon, Bruce
  • Assuntos: Chlamydia Infections - diagnosis ; Chlamydia trachomatis ; Chlamydia trachomatis - isolation & purification ; Correspondence ; Female ; Humans ; Immunoenzyme Techniques ; Infections ; Male ; Polymerase Chain Reaction ; Sexually transmitted diseases
  • É parte de: The Journal of infectious diseases, 1994-08, Vol.170 (2), p.500-501
  • Notas: istex:9910A56565DD939BF52F22CA099C188A11AB3FC6
    ark:/67375/HXZ-RKPWQ9GX-Q
    Reprints or correspondence: Dr. Richard L. Sweet. Dept. of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine. Magee-Womens Hospital. 300 Halket si., Pittsburgh. PA 15213
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  • Descrição: Chlamydia infection can often be asymptomatic in both male and female patients. There are similar to 4 million new cases of Chlamydia trachomatis infection annually. A sensitive, easy to use assay for C. trachomatis is apt to be recommended for populations at risk for sexually transmitted diseases (STDs). In an effort to evaluate the current C. trachomatis testing procedures in an inner-city public health setting, 203 male and female patients (consecutive) who presented to the Allegheny County Health Department's STD clinic were tested by three methods for C. trachomatis. Randomly collected specimens were assayed by EIA, culture, and polymerase chain reaction (PCR). Ten patients (4.9%) tested positive for C. trachomatis by EIA and 12 patients (5.9%) were positive by culture. By PCR analysis, C. trachomatis infections were detected in 14 patients (6.9%). These preliminary data show that a clinically important difference may exist between the currently used EIA screening assay and the recently released Roche PCR assay for C. trachomatis.
  • Editor: United States: The University of Chicago Press
  • Idioma: Inglês
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