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Cytochrome P450 (P450) isoforms expression, P450 concentration, monooxygenase activities, reactive oxygen species formation, lipid peroxidation, and glutathione content in wild catch carp and tench liver - influence of a two weeks exposure to phenobarbital

Klinger, W. ; Lupp, A. ; Barth, A. ; Karge, E. ; Knels, L. ; Kuhn, U. ; Müller, D. ; Schiller, F. ; Demme, U. ; Lindström-Seppä, P. ; Hänninen, O.

Experimental and toxicologic pathology (Print), 2001-02, Vol.52 (6), p.513-522 [Periódico revisado por pares]

Jena: Elsevier GmbH

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Título:
Cytochrome P450 (P450) isoforms expression, P450 concentration, monooxygenase activities, reactive oxygen species formation, lipid peroxidation, and glutathione content in wild catch carp and tench liver - influence of a two weeks exposure to phenobarbital
Autor: Klinger, W. ; Lupp, A. ; Barth, A. ; Karge, E. ; Knels, L. ; Kuhn, U. ; Müller, D. ; Schiller, F. ; Demme, U. ; Lindström-Seppä, P. ; Hänninen, O.
Assuntos: Animals ; Biological and medical sciences ; Carp ; Carps - metabolism ; Cytochrome P-450 Enzyme System - biosynthesis ; Dose-Response Relationship, Drug ; Environmental pollutants toxicology ; Enzyme Induction ; Female ; Fresh Water ; Glutathione ; Glutathione - metabolism ; GSH ; Isoenzymes - biosynthesis ; Lipid peroxidation ; Lipid Peroxidation - drug effects ; Liver ; Liver - drug effects ; Liver - metabolism ; Male ; Medical sciences ; Monooxygenation ; Oxygen formation ; P450 ; Peroxidation, lipid ; Phenobarbital ; Phenobarbital - pharmacology ; Reactive Oxygen Species - metabolism ; ROS and LPO formation ; Species Specificity ; Steroid Hydroxylases - metabolism ; Tench ; Toxicology ; Water
É parte de: Experimental and toxicologic pathology (Print), 2001-02, Vol.52 (6), p.513-522
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Carps, both sexes, 3 years old, weighing about 1 kg, and tenches of both sexes, 6 years old, weight about 250 g, were caught from a Thuringian lake without industrial pollution in November 1995 (fish without food uptake, water temperature at about 10°C) and kept for 2 weeks in basins with clean water and addition of 0, 0.1, 1.0 or 10.0 mg/l phenobarbital-Na (PB). The concentration of PB was controlled during and at the end of the exposure period. The animals were fed pellets, but no food uptake was observed. After 24–48 h in fresh water the fish were sacrificed and the following hepatic parameters were immediately determined biochemically: monooxygenase functions: cytochrome P450 (P450) content, ethylmorphine N-demethylation (EN), ethoxycoumarin O-deethylation (ECOD), ethoxyresorufin O-deethylation (EROD), 7-benzyloxy-4- methyl-coumarin O-debenzylation (BCDB); oxidase function indicators: microsomal Fe 2+/NADPH dependent hydrogen peroxide formation (H 20 2), microsomal Fe 2+/NADPH dependent luminol and lucigenin amplified chemiluminescence (LMCL, LCCL), microsomal Fe 2+/NADPH dependent lipid peroxide formation (LPO); oxidative state: lipid peroxidation products (TBARS) and GSH and GSSG. Additionally, the expression of three P450 isoforms, 1A1, 2B and 3A, was assessed immunohistochemically in tissue samples from brain, gill, heart, spleen, liver, gut and ovary of both fish species and in kidney of tenches. PB did not influence body or liver weights, but increased liver P450 concentration in both species by 50-100%, though not significantly. Carp: PB increased both EN and EROD significantly, but not ECOD and BCDB; H 20 2 and TBARS were enhanced significantly. LPO, LMCL and LCCL were not significantly influenced. Tench: PB increased all monooxygenase reactions (EN, ECOD, BCDB and EROD), though only significantly ECOD; H 20 2 was elevated only after treatment with 0.1 mg/1 PB, whereas LPO was decreased (!) after treatment by all three concentrations, though significantly only after 1.0 mg/l PB. LMCL was depressed (not significantly), but LCCL increased 5fold. TBARS were significantly enhanced. P450 1A1 sUbtype expression was concentration dependently elevated by PB in gill and liver of both fish and in the heart and kidney of tenches, P450 2B and 3A isoforms expression was induced in brain, gill, heart, liver and gut of both fish and in the kidney of tenches. In summary, the increased activities of the monooxygenase reactions tested and the elevated expression of all three P450 isoforms investigated in certain tissues indicate an induction of the P450 families 1, 2 and 3 by PB in fish.
Editor: Jena: Elsevier GmbH
Idioma: Inglês

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Cytochrome P450 (P450) isoforms expression, P450 concentration, monooxygenase activities, reactive oxygen species formation, lipid peroxidation, and glutathione content in wild catch carp and tench liver - influence of a two weeks exposure to phenobarbital

Klinger, W. ; Lupp, A. ; Barth, A. ; Karge, E. ; Knels, L. ; Kuhn, U. ; Müller, D. ; Schiller, F. ; Demme, U. ; Lindström-Seppä, P. ; Hänninen, O.

Jena: Elsevier GmbH

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