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Spontaneous Phosphorylation of the Receptor with High Affinity for IgE in Transfected Fibroblasts

Torigoe, Chikako ; Metzger, Henry

Biochemistry (Easton), 2001-04, Vol.40 (13), p.4016-4025 [Periódico revisado por pares]

United States: American Chemical Society

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Título:
Spontaneous Phosphorylation of the Receptor with High Affinity for IgE in Transfected Fibroblasts
Autor: Torigoe, Chikako ; Metzger, Henry
Assuntos: AFc receptors ; Amino Acid Sequence ; Animals ; CHO Cells ; Clone Cells ; Cricetinae ; Energy Transfer - genetics ; Enzyme Activation - genetics ; Fibroblasts - cytology ; Fibroblasts - enzymology ; Fibroblasts - immunology ; Fibroblasts - metabolism ; Interphase - genetics ; Membrane Microdomains - genetics ; Membrane Microdomains - metabolism ; Phosphoric Monoester Hydrolases - genetics ; Phosphoric Monoester Hydrolases - metabolism ; Phosphorylation ; Rats ; Receptors, IgE - biosynthesis ; Receptors, IgE - genetics ; Receptors, IgE - metabolism ; Spectrometry, Fluorescence ; src-Family Kinases - genetics ; src-Family Kinases - metabolism ; Temperature ; Transfection ; Tumor Cells, Cultured ; Tyrosine - metabolism
É parte de: Biochemistry (Easton), 2001-04, Vol.40 (13), p.4016-4025
Notas: istex:2F98BB660E236C46E121D63E454240BBAF818757
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Descrição: Receptors with high affinity for IgE, FcεRI, which had been transfected into Chinese hamster ovary fibroblasts exhibit an over 20-fold greater spontaneous phosphorylation at physiological temperatures than the same receptors on the widely studied rat mucosal mast cell line, RBL-2H3. This enhanced phosphorylation was not accounted for either by changes in the src-family kinase responsible for the phosphorylation, by reduced activity of phosphatases, or by spontaneous association of the receptors with microdomains. A variety of approaches failed to detect evidence for stable spontaneous aggregates of the receptor. Whereas the altered posttranslational glycosylation of the receptor's principal ectodomain we detected could promote transient spontaneous aggregation and explain the observed effect, other changes in the membrane milieu cannot be excluded. The functional consequences of such spontaneous phosphorylation are considered.
Editor: United States: American Chemical Society
Idioma: Inglês

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Spontaneous Phosphorylation of the Receptor with High Affinity for IgE in Transfected Fibroblasts

Torigoe, Chikako ; Metzger, Henry

United States: American Chemical Society

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