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Purification of African trypanosomes can cause biochemical changes in the parasites

Lonsdale-Eccles, J.D ; Grab, D.J

The Journal of Protozoology, 1987-11, Vol.34 (4), p.405-408 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Purification of African trypanosomes can cause biochemical changes in the parasites
Autor: Lonsdale-Eccles, J.D ; Grab, D.J
Assuntos: ADENOSINE TRIPHOSPHATE ; Adenosine Triphosphate - metabolism ; ADENOSINTRIFOSFATO ; Animals ; BIOCHEMISTRY ; Biochemistry. Physiology. Immunology. Molecular biology ; BIOCHIMIE ; Biological and medical sciences ; BIOQUIMICA ; BLOOD ; Centrifugation ; Centrifugation, Isopycnic ; Chromatography, DEAE-Cellulose ; Endopeptidases - metabolism ; EPURATION ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Phospholipases - metabolism ; Protozoa ; PURIFICACION ; PURIFICATION ; SANG ; SANGRE ; TRYPANOSOMA ; TRYPANOSOMA BRUCEI ; Trypanosoma brucei brucei - enzymology ; Trypanosoma brucei brucei - isolation & purification ; Trypanosoma brucei brucei - metabolism ; TRYPANOSOMA CONGOLENSE ; Trypanosoma congolense - enzymology ; Trypanosoma congolense - isolation & purification ; Trypanosoma congolense - metabolism ; Trypanosoma vivax ; Variant Surface Glycoproteins, Trypanosoma - metabolism
É parte de: The Journal of Protozoology, 1987-11, Vol.34 (4), p.405-408
Notas: 881066988
L72
We gratefully acknowledge George W. N. Mpimbaza and Yasmin Verjee for their excellent technical help during this study and Lucy Thairo for typing this manuscript. This is ILRAD publication number 494.
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Descrição: Bloodstream forms of African trypanosomes are routinely purified from blood components by a combination of centrifugation and chromatography on DEAE cellulose at pH 8.0. Here we report that the nonphysiological conditions used for DEAE chromatography of the parasites result in changes in the ATP levels of the trypanosomes and an enhanced release from the parasites of proteins such as variable surface glycoprotein, peptidase, and phospholipase. Some of these changes can be reduced by the addition of nucleosides to the elution buffer and, after the elution of the parasites, by immediate readjustment of the external pH to the normal physiological level of blood (pH 7.4).
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Purification of African trypanosomes can cause biochemical changes in the parasites

Lonsdale-Eccles, J.D ; Grab, D.J

Oxford, UK: Blackwell Publishing Ltd

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