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Epitope Mapping of the Anti-Urokinase Monoclonal Antibody 5B4 by Isolated Domains of Urokinase

Corti, Angelo ; Sarubbi, Edoardo ; Soffientini, Adolfo ; Nolli, M Luisa ; Zanni, Arturo ; Galimberti, Marina ; Parenti, Franco ; Cassani, Giovanni

Thrombosis and haemostasis, 1989-11, Vol.62 (3), p.934-939 [Periódico revisado por pares]

Stuttgart: Schattauer GmbH

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Título:
Epitope Mapping of the Anti-Urokinase Monoclonal Antibody 5B4 by Isolated Domains of Urokinase
Autor: Corti, Angelo ; Sarubbi, Edoardo ; Soffientini, Adolfo ; Nolli, M Luisa ; Zanni, Arturo ; Galimberti, Marina ; Parenti, Franco ; Cassani, Giovanni
Assuntos: Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Antibodies, Monoclonal - analysis ; Biological and medical sciences ; Enzymes and enzyme inhibitors ; Epitopes - analysis ; Fibrinolytic Agents - immunology ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrolases ; Molecular Sequence Data ; Original Article ; Peptide Fragments - analysis ; Plasminogen Activators - immunology ; Plasminogen Activators - metabolism ; Receptors, Cell Surface - metabolism ; Receptors, Urokinase Plasminogen Activator ; Urokinase-Type Plasminogen Activator - immunology ; Urokinase-Type Plasminogen Activator - metabolism
É parte de: Thrombosis and haemostasis, 1989-11, Vol.62 (3), p.934-939
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Summary The amino terminal fragment (ATF) of urokinase-type plasminogen activator (uPA) is a degradation product comprising the entire growth factor-like and kringle domains. It has been previously shown that ATF is able to bind to the u-PA receptor through the growth factor-like domain and that the anti u-PA monoclonal antibody 5B4 (Mab 5B4) binds to ATF preventing u-PA receptor binding. To localize more precisely the epitope recognized by Mab 5B4, ATF was subfragmented by controlled enzymatic proteolysis with V8 protease. Three subfragments of 4,000 M r (F-4k), 11,000 M r (F-llk) and 12,0 M r (F-12k) were purified from the reaction mixture and characterized. SDS-PAGE under reducing and non-reducing conditions, N-terminal aminoacid sequence analysis and C-terminal aminoacid analysis of each fragment indicate that F-4k and F-llk correspond to intact growth factor-like domain and kringle domain (residues 4-43 and 44-135 respectively) while F-12k corresponds to the kringle. domain cleaved in the first loop at the glu52-gly53 bond. By Western blot and competitive binding experiments we show that Mab 5B4 recognizes an epitope located on the kringle domain of u-PA and that the binding is strongly reduced when the kringle contains an additional cleavage in its first loop. Since the receptor binding site of u-PA has been previously shown to be located on the growth factor-like domain, Mab 5B4 inhibits the binding of uPA to its cellular receptor likely by steric hindrance. Besides the proven utility in epitope localization of anti u-PA monoclonal antibodies, these u-PA fragments may represent powerful tools for studies of structure-function relationship of u-PA.
Editor: Stuttgart: Schattauer GmbH
Idioma: Inglês

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Epitope Mapping of the Anti-Urokinase Monoclonal Antibody 5B4 by Isolated Domains of Urokinase

Corti, Angelo ; Sarubbi, Edoardo ; Soffientini, Adolfo ; Nolli, M Luisa ; Zanni, Arturo ; Galimberti, Marina ; Parenti, Franco ; Cassani, Giovanni

Stuttgart: Schattauer GmbH

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