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Determination of thiobarbituric acid reactive substances in microencapsulated products
Hu, Zhixiong ; Zhong, Qixin
Food chemistry, 2010-12, Vol.123 (3), p.794-799
[Periódico revisado por pares]
Kidlington: Elsevier Ltd
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Título:
Determination of thiobarbituric acid reactive substances in microencapsulated products
Autor:
Hu, Zhixiong
;
Zhong, Qixin
Assuntos:
accuracy
;
antioxidant activity
;
Biological and medical sciences
;
butanol
;
coatings
;
Complete dissolution
;
Encapsulation
;
Fish
;
fish oils
;
food composition
;
Food industries
;
food processing
;
food processing quality
;
Foods
;
Fundamental and applied biological sciences. Psychology
;
isopropyl alcohol
;
Lipid oxidation
;
lipid peroxidation
;
Lipids
;
Microencapsulation
;
Polyunsaturated fatty acids
;
precision
;
reliability
;
repeatability
;
Solvents
;
TBARS
;
Thiobarbituric acid
;
thiobarbituric acid-reactive substances
;
Zein
É parte de:
Food chemistry, 2010-12, Vol.123 (3), p.794-799
Notas:
http://dx.doi.org/10.1016/j.foodchem.2010.05.012
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição:
Food samples are usually extracted or distillated before quantification of thiobarbituric acid reactive substances (TBARS) for evaluation of secondary lipid oxidation, which is a challenge for microencapsulation projects with limited sample quantities. Our approach was to use a ternary solvent mixture of 1-butanol, isopropanol, and 0.5 M HCl (in water) at a proportion of 2:2:1 (v/v/v) to completely dissolve microcapsules of fish oil. The solvent system allowed the construction of highly linear standard curves with good sensitivity: 0.4–13 μM 1,1,3,3,-tetramethoxypropane as a TBA reactive standard with a R 2 value of 99.86%. For a sample of fish oil coated by corn zein, only 40 mg of the sample was needed to obtain reliable and repeatable TBARS values. Further, several common encapsulation materials gave low interferences to the assay. The proposed approach, with the above qualities along with good precision and accuracy, may be applicable to a variety of relevant bioactive compounds and encapsulation materials.
Editor:
Kidlington: Elsevier Ltd
Idioma:
Inglês
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