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Enzymatic Dehydrogenation of Testosterone Coupled to Pyruvate Reduction in a Two‐Phase System

Cremonesi, Pietro ; Carrea, Giacomo ; Ferrara, Luigi ; Antonini, Eraldo

European journal of biochemistry, 1974-05, Vol.44 (2), p.401-405 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Enzymatic Dehydrogenation of Testosterone Coupled to Pyruvate Reduction in a Two‐Phase System
Autor: Cremonesi, Pietro ; Carrea, Giacomo ; Ferrara, Luigi ; Antonini, Eraldo
Assuntos: Acetates ; Androstenedione - metabolism ; Butanols ; Emulsions ; Esters ; Hydrogen-Ion Concentration ; Hydroxysteroid Dehydrogenases ; Kinetics ; L-Lactate Dehydrogenase ; Methods ; NAD ; Pyruvate Dehydrogenase Complex ; Solvents ; Temperature ; Testosterone - metabolism ; Water
É parte de: European journal of biochemistry, 1974-05, Vol.44 (2), p.401-405
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Following previous work, the enzymatic dehydrogenation of testosterone into 4‐androstene‐3,17‐dione is carried out in water and organic solvent system. The organic solvent contains the steroid and the water phase contains the β‐hydroxysteroid dehydrogenase together with pyruvate and lactate dehydrogenase. In such a system, the reduced coenzyme formed by dehydrogenation of the steroid is oxidized again by pyruvate and the conversion of testosterone is essentially stoichiometric with the pyruvate added. The effect of several variables on the equilibrium and kinetic behaviour of the system has been analyzed. The results obtained provide another good example of the advantages which may be obtained in carrying out enzyme reactions with poorly water‐soluble substrates in emulsions of water and organic solvents.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Enzymatic Dehydrogenation of Testosterone Coupled to Pyruvate Reduction in a Two‐Phase System

Cremonesi, Pietro ; Carrea, Giacomo ; Ferrara, Luigi ; Antonini, Eraldo

Oxford, UK: Blackwell Publishing Ltd

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