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Role of alpha 7 Nicotinic Acetylcholine Receptor in Calcium Signaling Induced by Prion Protein Interaction with Stress-inducible Protein 1

Beraldo, Flavio H ; Arantes, Camila P ; Santos, Tiago G ; Queiroz, Nicolle GT ; Young, Kirk ; Rylett, RJane ; Markus, Regina P ; Prado, Marco AM ; Martins, Vilma R

The Journal of biological chemistry, 2010-11, Vol.285 (47), p.36542-36550 [Periódico revisado por pares]

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  • Título:
    Role of alpha 7 Nicotinic Acetylcholine Receptor in Calcium Signaling Induced by Prion Protein Interaction with Stress-inducible Protein 1
  • Autor: Beraldo, Flavio H ; Arantes, Camila P ; Santos, Tiago G ; Queiroz, Nicolle GT ; Young, Kirk ; Rylett, RJane ; Markus, Regina P ; Prado, Marco AM ; Martins, Vilma R
  • É parte de: The Journal of biological chemistry, 2010-11, Vol.285 (47), p.36542-36550
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-1
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  • Descrição: The prion protein (PrPC) is a conserved glycosylphosphatidylinositol-anchored cell surface protein expressed by neurons and other cells. Stress-inducible protein 1 (STI1) binds PrPC extracellularly, and this activated signaling complex promotes neuronal differentiation and neuroprotection via the extracellular signal-regulated kinase 1 and 2 (ERK1/2) and cAMP-dependent protein kinase 1 (PKA) pathways. However, the mechanism by which the PrPC-STI1 interaction transduces extracellular signals to the intracellular environment is unknown. We found that in hippocampal neurons, STI1-PrPC engagement induces an increase in intracellular Ca2+ levels. This effect was not detected in PrPC-null neurons or wild-type neurons treated with an STI1 mutant unable to bind PrPC. Using a best candidate approach to test for potential channels involved in Ca2+ influx evoked by STI1-PrPC, we found that alpha -bungarotoxin, a specific inhibitor for alpha 7 nicotinic acetylcholine receptor ( alpha 7nAChR), was able to block PrPC-STI1-mediated signaling, neuroprotection, and neuritogenesis. Importantly, when alpha 7nAChR was transfected into HEK 293 cells, it formed a functional complex with PrPC and allowed reconstitution of signaling by PrPC-STI1 interaction. These results indicate that STI1 can interact with the PrPC. alpha 7nAChR complex to promote signaling and provide a novel potential target for modulation of the effects of prion protein in neurodegenerative diseases.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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